Author | Nisimura, L. M. | |
Author | Bousquet, P. | |
Author | Muccillo, F. | |
Author | Tibiricá, E. | |
Author | Garzoni, L. R. | |
Access date | 2020-11-27T19:38:54Z | |
Available date | 2020-11-27T19:38:54Z | |
Document date | 2020 | |
Citation | NISIMURA, L. M. et al. Tyrosine hydroxylase and b2-adrenergic receptor expression in leukocytes of spontaneously hypertensive rats: putative peripheral markers of central sympathetic activity. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 53, n. 12, e9615, 2020. | pt_BR |
ISSN | 1414-431X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44665 | |
Language | eng | pt_BR |
Publisher | Associação Brasileira de Divulgação Científica | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Ataque Isquêmico Transitório | pt_BR |
Subject in Portuguese | Doenças do Sistema Nervoso Autônomo | pt_BR |
Subject in Portuguese | Leucócitos | pt_BR |
Subject in Portuguese | Clonidina | pt_BR |
Subject in Portuguese | Ratos Endogâmicos SHR | pt_BR |
Title | Tyrosine hydroxylase and b2-adrenergic receptor expression in leukocytes of spontaneously hypertensive rats: putative peripheral markers of central sympathetic activity | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/1414-431X20209615 | |
Abstract | The sympathetic nervous system (SNS) plays a fundamental role in the pathophysiology of cardiovascular diseases, including
primary arterial hypertension. In this study, we aimed to investigate whether the expression of the rate-limiting enzyme in
catecholamine synthesis, tyrosine hydroxylase (TH), and the b2-adrenergic receptor (b2-AR) in immune cells from peripheral
blood, reflect central SNS activity in spontaneously hypertensive rats (SHR). TH expression in the lower brainstem and adrenal
glands and b2-AR expression in the lower brainstem were analyzed by western blot analyses. In the leukocytes, TH and b2-AR
expression was evaluated by flow cytometry before and after chronic treatment with the centrally-acting sympathoinhibitory drug
clonidine. Western blot analyses showed increased TH and b2-AR expression in the lower brainstem and increased TH in
adrenal glands from SHR compared to normotensive Wistar Kyoto rats (WKY). Lower brainstem from SHR treated with
clonidine presented reduced TH and b2-AR levels, and adrenal glands had decreased TH expression compared to SHR treated
with vehicle. Flow cytometry showed that the percentage of leukocytes that express b2-AR is higher in SHR than in WKY.
However, the percentage of leukocytes that expressed TH was higher in WKY than in SHR. Moreover, chronic treatment with
clonidine normalized the levels of TH and b2-AR in leukocytes from SHR to similar levels of those of WKY. Our study
demonstrated that the percentage of leukocytes expressing TH and b2-AR was altered in arterial hypertension and can be
modulated by central sympathetic inhibition with clonidine treatment. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigac¸ão Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Strasbourg. Faculty of Medicine. Department of Pharmacology. Strasbourg, France. | pt_BR |
Affilliation | Ministério da Saúde. Instituto Nacional de Cardiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil / Ministério da Saúde. Instituto Nacional de Cardiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Brain stem | pt_BR |
Subject | Sympathetic nervous system | pt_BR |
Subject | Leukocytes | pt_BR |
Subject | Spontaneously hypertensive rats | pt_BR |
Subject | Clonidine | pt_BR |
DeCS | Ataque Isquêmico Transitório | pt_BR |
DeCS | Doenças do Sistema Nervoso Autônomo | pt_BR |
DeCS | Ratos Endogâmicos SHR | pt_BR |