Author | Genteluci, Gabrielle Limeira | |
Author | Gomes, Daniela Betzler Cardoso | |
Author | Pereira, Daniella Cristina Rodrigues | |
Author | Neves, Marta de Campos | |
Author | Souza, Maria José de | |
Author | Rangel, Karyne | |
Author | Villas Bôas, Maria Helena Simões | |
Access date | 2020-11-12T20:57:37Z | |
Available date | 2020-11-12T20:57:37Z | |
Document date | 2020 | |
Citation | GENTELUCI, Gabrielle Limeira et al. Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells. Brazilian Journal of Microbiology, São Paulo, v. 51, p. 657–664, mar. 2020. | pt_BR |
ISSN | 1517-8382 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44390 | |
Description | Daniella Pereira - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento do vinculo com a Fiocruz Brasília. | pt_BR |
Language | eng | pt_BR |
Publisher | Sociedade Brasileira de Microbiologia | pt_BR |
Rights | open access | pt_BR |
MeSH | Acinetobacter baumannii | pt_BR |
MeSH | Drug Resistance, Microbial | pt_BR |
MeSH | Biofilms | pt_BR |
Title | Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells | pt_BR |
Type | Article | pt_BR |
DOI | 10.1007/s42770-020-00252-x | |
Abstract | Acinetobacter baumannii has been associated with antimicrobial resistance and ability to form biofilms. Furthermore, its adherence
to host cells is an important factor to the colonization process. Therefore, this study intended to identify some virulence
factors that can explain the success of A. baumannii in causing nosocomial infections. We studied 92 A. baumannii isolates
collected from hospitals in Rio de Janeiro, Brazil. Isolates were identified and the susceptibility to antimicrobials was determined.
Oxacilinase type β-lactamase encoding genes were amplified by polymerase chain reaction, and genetic diversity was investigated
by pulsed-field gel electrophoresis (PFGE). In addition, biofilm formation on polystyrene plates using crystal violet
staining was quantified, and adherence to human cell lines was evaluated. Eighty-six isolates were multidrug-resistant, of which
93% were carbapenem-resistant. All isolates had the blaOXA-51 gene and 94% had the blaOXA-23 gene, other searched blaOXA
genes were not detected. PFGE typing showed two predominant clones, and biofilm production was observed in 79%of isolates.
A. baumannii isolates adhered better to HEp-2 cell compared with A-549 cell. Clones A, B, E, and F showed a significantly
increased adherence to HEp-2 compared with adherence to A-549 cell. Our findings revealed that A. baumannii isolates had high
frequencies of resistance to antimicrobial agents, ability to form biofilm, and capacity to adhere to HEp-2 cells. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Programa de Pós-Graduação em Vigilância em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Programa de Pós-Graduação em Vigilância em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Federal Hospital of State Servers. Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Acinetobacter baumannii | pt_BR |
Subject | Antimicrobial resistance | pt_BR |
Subject | PCR | pt_BR |
Subject | PFGE | pt_BR |
Subject | Biofilm | pt_BR |
Subject | Adherence to cell | pt_BR |
DeCS | Acinetobacter baumannii | pt_BR |
DeCS | Resistência Microbiana a Medicamentos | pt_BR |
DeCS | Biofilmes | pt_BR |