Author | Gomes, Yago | |
Author | Caterino-de-Araujo, Adele | |
Author | Campos, Karoline | |
Author | Gonçalves, Maria Gisele | |
Author | Leite, Ana Claudia | |
Author | Lima, Marco Antonio | |
Author | Araújo, Abelardo | |
Author | Silva, Marcus Tulius | |
Author | Espíndola, Otávio | |
Access date | 2020-10-31T18:07:41Z | |
Available date | 2020-10-31T18:07:41Z | |
Document date | 2020 | |
Citation | GOMES, Yago et al. Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection. Viruses, v. 12, n. 9, p. 1-15, Sept. 2020. | pt_BR |
ISSN | 1999-4915 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44292 | |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Title | Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/v12090981 | |
Abstract | Laboratory diagnosis of human T-lymphotropic viruses (HTLV) 1 and 2 infection is performed by serological screening and further confirmation with serological or molecular assays. Thus, we developed a loop-mediated isothermal nucleic acid amplification (LAMP) assay for the detection of HTLV-1/2 in blood samples. The sensitivity and accuracy of HTLV-1/2 LAMP were defined with DNA samples from individuals infected with HTLV-1 (n = 125), HTLV-2 (n = 19), and coinfected with HIV (n = 82), and compared with real-time polymerase chain reaction (qPCR) and PCR-restriction fragment length polymorphism (RFLP). The overall accuracy of HTLV-1/2 LAMP (95% CI 74.8-85.5%) was slightly superior to qPCR (95% CI 69.5-81.1%) and similar to PCR-RFLP (95% CI 79.5-89.3%). The sensitivity of LAMP was greater for HTLV-1 (95% CI 83.2-93.4%) than for HTLV-2 (95% CI 43.2-70.8%). This was also observed in qPCR and PCR-RFLP, which was associated with the commonly lower HTLV-2 proviral load. All molecular assays tested showed better results with samples from HTLV-1/2 mono-infected individuals compared with HIV-coinfected patients, who present lower CD4 T-cell counts. In conclusion, HTLV-1/2 LAMP had similar to superior performance than PCR-based assays, and therefore may represent an attractive alternative for HTLV-1/2 diagnosis due to reduced working time and costs, and the simple infrastructure needed. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Adolfo Lutz Institute. Immunology Center. Laboratory of HTLV Research. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Adolfo Lutz Institute. Immunology Center. Laboratory of HTLV Research. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Adolfo Lutz Institute. Immunology Center. Laboratory of HTLV Research. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | HTLV-1 | pt_BR |
Subject | HTLV-2 | pt_BR |
Subject | LAMP | pt_BR |
Subject | Confirmatory diagnosis | pt_BR |