Author | Almeida, Viviane de Sousa Moreira | |
Author | Azevedo, Jailton | |
Author | Leal, Helena Ferreira | |
Author | Queiroz, Artur Trancoso Lopo de | |
Author | Silva Filho, Hermes Pedreira da | |
Author | Reis, Joice Neves | |
Access date | 2020-10-31T12:19:04Z | |
Available date | 2020-10-31T12:19:04Z | |
Document date | 2020 | |
Citation | ALMEIDA, Viviane de Sousa Moreira et al. Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome. Plos One, p. 1-17, Sept. 29, 2020. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44289 | |
Sponsorship | CAPES (Coordenação de Aperfeiçoamento de
Pessoal de Nível Superior- VSMA), FAPESB
(Fundação de Amparo à Pesquisa do Estado da
Bahia-HFL, JNR), INPRA (Instituto Nacional de
Pesquisa em Resistência Antimicrobiana-JNR),
CNPq (Conselho Nacional de Desenvolvimento
Científico e Tecnológico), and FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do
Sul) for their financial support. ATLQ acknowledges
financial support from the program Inova Fiocruz
(Project number VPPIS-001-FIO18). | pt_BR |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Antibacterianos | pt_BR |
Subject in Portuguese | Resistência Microbiana a Medicamentos | pt_BR |
Subject in Portuguese | Microbiota | pt_BR |
Subject in Portuguese | Periodontite | pt_BR |
Subject in Portuguese | Doenças periodontais | pt_BR |
Title | Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0239664 | |
Abstract | This study aims to describe the oral microbiome diversity and prevalence of ARGs in periodontal
health and disease.
Background
The human oral cavity harbors a complex microbial community known as the oral microbiome.
These organisms are regularly exposed to selective pressures, such as the usage of
antibiotics, which drive evolution and acquisition of antibiotic resistance genes (ARGs).
Resistance among oral bacteria jeopardizes not only antibiotic therapy for oral infections,
but also extra-oral infections caused by bacterial translocation.
Methods
We carried out a cross-sectional investigation. Saliva and subgingival plaque samples were
collected during a clinical exam. 16S rRNA gene sequencing was performed to assess
microbial diversity. Resistance genes were identified through PCR assays.
Results
Of the 110 participants, only 22.7% had healthy periodontium, while the majority was diagnosed
with gingivitis (55.4%) and chronic periodontitis (21.8%). The composition of the oral
microbiota differed from healthy and diseased samples, being Streptococcus spp. and
Rothia spp. predominant in periodontal disease. Regarding ARGs, 80 (72.7%) samples
were positive for at least one of genes screened, erm being the most frequent variant
(58.2%), followed by blaTEM (16.4%), mecA (2.7%), pbp2b and aac(6 ’) (1.8%). Neither
genes coding resistance to carbapenems nor metronidazole were detected.
Conclusions
Our findings indicate that there are no significant differences in terms of taxonomic enrichment
between healthy and diseased oral microbiomes. However, samples retrieved from
healthy patients had a more diverse microbial community, whereas diseased samples have lower taxonomic diversity. We have also identified clinically relevant ARGs, providing baseline
information to guide antibiotic prescription in dentistry. | pt_BR |
Affilliation | Federal University of Bahia. School of Pharmacy. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Federal University of Recôncavo da Bahia. Health Science Center. Santo Antônio de Jesus, BA, Brazil. | pt_BR |
Affilliation | Federal University of Bahia. School of Pharmacy. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Subject | Anti-Bacterial Agents | pt_BR |
Subject | Drug Resistance, Microbial | pt_BR |
Subject | Microbiota | pt_BR |
Subject | Periodontitis | pt_BR |
Subject | Periodontal Diseases | pt_BR |