Author | Matos, Denise Cristina Souza | |
Author | Marcovistz, Rugimar | |
Author | Cabello, Pedro Herman | |
Author | Georgini, Ricardo Amaral | |
Author | Sakauchi, Dirce | |
Author | Silva, Luciana Leite da | |
Access date | 2020-10-26T19:08:55Z | |
Available date | 2020-10-26T19:08:55Z | |
Document date | 2002 | |
Citation | MATOS, Denise Cristina Souza et al. Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 6, p. 909-913, Sept. 2002. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44211 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Vacina contra difteria-tétano | pt_BR |
Subject in Portuguese | Vacina contra difteria-tétano-coqueluche | pt_BR |
Subject in Portuguese | Teste de inibição de ligação de toxinas | pt_BR |
Subject in Portuguese | Tétano | pt_BR |
Subject in Portuguese | Controle de qualidade | pt_BR |
Subject in Portuguese | Soroneutralização | pt_BR |
Title | Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762002000600030 | |
Abstract | Samples from 20 lots of diphtheria-tetanus (adult use dT) vaccine and from 20 lots of diphtheria-tetanuspertussis (DTP) vaccine were used to standardize and validate the in vitro toxin binding inhibition (ToBI) test for
the immunogenicity test of the tetanus component. The levels of tetanus antitoxin obtained by ToBI test were compared to those obtained using the toxin neutralization (TN) test in mice routinely employed to perform the quality
control of the tetanus component in adsorbed vaccines. The results ranged from 1.8 to 3.5 IU/ml for dT and 2 to 4 IU/
ml for DTP by ToBI test and 1.4 to 3 IU/ml for dT and 1.8 to 3.5 IU/ml for DTP by TN in mice. These results were
significantly correlated. From this study, it is concluded that the ToBI test is an alternative to the in vivo neutralization procedure in the immunogenicity test of the tetanus component in adsorbed vaccines. A substantial refinement
and a reduction in use of animals can be achieved. | pt_BR |
Affilliation | Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil. | pt_BR |
Subject | Diphtheria-tetanus vaccine | pt_BR |
Subject | Diphtheria-tetanus-pertussis vaccine | pt_BR |
Subject | Toxin binding inhibition test | pt_BR |
Subject | Tetanus | pt_BR |
Subject | Quality control | pt_BR |
Subject | Seroneutralization | pt_BR |
e-ISSN | 1678-8060 | |