Author | Ferreira, Rosa Maria Carvalho | |
Author | Saad, Maria Helena Féres | |
Author | Silva, Marlei Gomes da | |
Author | Fonseca, Leila de Souza | |
Access date | 2020-10-26T14:20:04Z | |
Available date | 2020-10-26T14:20:04Z | |
Document date | 2002 | |
Citation | FERREIRA, Rosa Maria Carvalho et al. Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 5, p. 725-729, July 2002. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44194 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Tuberculose | pt_BR |
Subject in Portuguese | Micobactérias não tuberculosas | pt_BR |
Subject in Portuguese | Acquired immunodeficiency | pt_BR |
Subject in Portuguese | Identificação bioquímica | pt_BR |
Subject in Portuguese | Rio de Janeiro | pt_BR |
Subject in Portuguese | Brasil | pt_BR |
Title | Non-tuberculous Mycobacteria I: One Year Clinical Isolates Identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in Pre Highly Active Antiretroviral Therapy Era | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762002000500024 | |
Abstract | The aim of this study was to determine the prevalence of non-tuberculous mycobacteria (NTM) isolates at
University Hospital, Reference Center for Aids in Rio de Janeiro, Brazil, during one year. We used standard biochemical tests for species identification and IS1245 PCR amplification was applied as a Mycobacterium avium
specific identification marker. Four hundred and four specimens from 233 patients yielded acid-fast bacilli growth.
M. tuberculosis was identified in 85% of the patients and NTM in 15%. NTM disseminated infection was a common
event correlated with human immunodeficiency virus (HIV) infected patients and only in HIV negative patients the
source of NTM was non sterile site. M. avium complex (MAC) was biochemically identified in 57.8% (49/83) of NTM
isolates, most of them from sterile sites (75.5%), and in 94% (46/49) the IS 1245 marker specific for M. avium was
present. Twenty NTM strains showed a MAC biochemical pattern with the exception of a urease-positive (99% of
MAC are urease-negative), however IS1245 was detected in 96% of the strains leading to their identification as M.
avium. In this group differences in NTM source was not significant. The second most frequently isolated NTM was
identified as M. scrofulaceum (7.2%), followed by M. terrae (3.6%), M. gordonae (2.4%), M. chelonae (1.2%), M.
fortuitum (1.2%) and one strain which could not be identified. All were IS1245 negative except for one strain
identified as M. scrofulaceum. It is interesting to note that non-sterile sites were the major source of these isolates
(92.8%). Our finding indicated that M. avium is still the major atypical species among in the MAC isolates recovered from Brazilian Aids patients without highty active antiretroviral therapy schema. Some discrepancies were
seen between the identification methods and further investigations must be done to better characterize NTM isolates
using other phenotypic and genotypic methods. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Laboratório de Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Non-tuberculous mycobacteria | pt_BR |
Subject | Tuberculosis | pt_BR |
Subject | Acquired immunodeficiency | pt_BR |
Subject | IS1245 | pt_BR |
Subject | Biochemical identification | pt_BR |
Subject | Rio de Janeiro | pt_BR |
Subject | Brazil | pt_BR |
e-ISSN | 1678-8060 | |