Author | Bertho, Álvaro L. | |
Author | Santiago, Marta A. | |
Author | Coutinho, Sérgio G. | |
Access date | 2020-10-19T18:51:06Z | |
Available date | 2020-10-19T18:51:06Z | |
Document date | 2000 | |
Citation | BERTHO, Álvaro L.; SANTIAGO, Marta A.; COUTINHO, Sérgio G. Flow Cytometry in the Study of Cell Death. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 3, p. 429-433, May/June 2000. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44068 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Apoptose | pt_BR |
Subject in Portuguese | Citometria de fluxo | pt_BR |
Subject in Portuguese | Morte celular | pt_BR |
Subject in Portuguese | Necrose | pt_BR |
Title | Flow Cytometry in the Study of Cell Death | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762000000300020 | |
Abstract | In this report we present a concise review concerning the use of flow cytometric methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis. The
applications of these techniques to clinical and basic research are also considered. The following cell
features are useful to characterize the mode of cell death: (1) activation of an endonuclease in apoptotic
cells results in extraction of the low molecular weight DNA following cell permeabilization, which, in
turn, leads to their decreased stainability with DNA-specific fluorochromes. Measurements of DNA
content make it possible to identify apoptotic cells and to recognize the cell cycle phase specificity of
apoptotic process; (2) plasma membrane integrity, which is lost in necrotic but not in apoptotic cells; (3)
the decrease in forward light scatter, paralleled either by no change or an increase in side scatter,
represent early changes during apoptosis. The data presented indicate that flow cytometry can be applied to basic research of the molecular and biochemical mechanisms of apoptosis, as well as in the
clinical situations, where the ability to monitor early signs of apoptosis in some systems may be predictive for the outcome of some treatment protocols. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Imunidade Celular e Humoral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Imunidade Celular e Humoral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Imunidade Celular e Humoral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Apoptosis | pt_BR |
Subject | Flow cytometry | pt_BR |
Subject | Cell death | pt_BR |
Subject | Necrosis | pt_BR |
e-ISSN | 1678-8060 | |