Author | Bezerra, Camila Fonseca | |
Author | Alencar Júnior, José Geraldo de | |
Author | Honorato, Rosilaine de Lima | |
Author | Santos, Antonia Thassya Lucas dos | |
Author | Silva, Josefa Carolaine Pereira da | |
Author | Silva, Taís Gusmão da | |
Author | Leal, Antonio Linkoln Alves Borges | |
Author | Freitas, Thiago Sampaio de | |
Author | Vieira, Thiago Adler Tavares | |
Author | Rocha, Janaína Esmeraldo | |
Author | Sales, Debora Lima | |
Author | Barbosa Filho, José Maria | |
Author | Sousa, Gabriela Ribeiro de | |
Author | Pinheiro, Allyson Pontes | |
Author | Ribeiro Filho, Jaime | |
Author | Coutinho, Henrique Douglas Melo | |
Author | Braga, Maria Flaviana Bezerra Morais | |
Author | Silva, Teresinha Gonçalves da | |
Access date | 2020-09-23T11:54:40Z | |
Available date | 2020-09-23T11:54:40Z | |
Document date | 2020 | |
Citation | BEZERRA, Camila Fonseca et al. Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole. Membranes, 2020. | pt_BR |
ISSN | 2077-0375 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/43562 | |
Description | Jaime Ribeiro-Filho. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. 1 Department of Pharmaceutical Sciences, Federal University of Pernambuco-UFPE,
Recife-PE 50670-901, Brazil; camilawasidi@gmail.com
2 Department of Pharmacy, Federal University of Ceará-UFC, Fortaleza-CE 60020-181, Brazil;
junioralencar727@gmail.com
3 Department of Biological Sciences, Regional University of Cariri-URCA, Crato-CE 63105-010, Brazil;
rosilainehonorato@gmail.com (R.d.L.H.); thassyalucas@hotmail.com (A.T.L.d.S.);
carol.bio1881@outlook.com (J.C.P.d.S.); taisgusmao96@gmail.com (T.G.d.S.);
allysson.ponheiro@urca.br (A.P.P.); flavianamoraisb@yahoo.com.br (M.F.B.M.-B.)
4 Department of Biological Chemistry, Regional University of Cariri-URCA, Crato-CE 63105-010, Brazil;
antoniolinkoln@hotmail.com (A.L.A.B.L.); thiagocrato@hotmail.com (T.S.d.F.);
thiago.a.t.vieira@gmail.com (T.A.T.V.); janainaesmeraldo@gmail.com (J.E.R.);
debora.lima.sales@gmail.com (D.L.S.)
5 Department of Pharmacy, Federal University of Paraíba-UFPB, João Pessoa-PB 58051-900, Brazil;
jbarbosa@ltf.ufpb.br (J.M.B.F.); grsousafarm@gmail.com (G.R.d.S.)
6 Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador-BA 40296-710, Brazil;
jaimeribeirofilho@gmail.com
7 Departament of Antibiotics, Federal University of Pernambuco-UFPE, Recife-PE 50670-901, Brazil;
teresinha100@gmail.com | pt_BR |
Sponsorship | National Council for Scientific Development and Technological (CNPq) and
the Coordination for the Improvement of Level Personnel Superior (CAPES), for supporting the work. | pt_BR |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Lipossomas | pt_BR |
Subject in Portuguese | Fluconazol | pt_BR |
Subject in Portuguese | Candida | pt_BR |
Subject in Portuguese | Anticorpos Antifúngicos | pt_BR |
Title | Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole | pt_BR |
Type | Article | pt_BR |
Abstract | Background: Infections by Candida species represent a serious threat to the health of
immunocompromised individuals. Evidence has indicated that nerolidol has significant antifungal
properties. Nonetheless, its use is restricted due to a low water solubility and high photosensitivity.
The incorporation into liposomes may represent an e cient alternative to improve the physicochemical
and biopharmaceutical properties of this compound. The present study aimed to characterize the
antifungal properties of liposomal nerolidol, alone or in combination with fluconazole. Of note, this is
the first study reporting the antifungal activity of liposomal nerolidol and its potentiating e ect in
association with fluconazole. (2) Methods: The Inhibitory Concentration 50%-IC50 and minimum
fungicide concentrations (MFC) of the substances against Candida albicans (CA), Candida tropicalis
(CT), and Candida krusei (CK) were established by subculture in a solid medium. To evaluate the
antifungal-enhancing e ect, the MFC of fluconazole was determined in the presence or absence of
subinhibitory concentrations of nerolidol (free or liposomal). The analysis of fungal dimorphism
was performed through optical microscopy and the characterization of liposomes was carried out
considering the vesicular size, polydispersion index, and zeta medium potential, in addition to a
scanning electron microscopy analysis. (3) Results: The physicochemical characterization revealed
that liposomes were obtained as homogenous populations of spherical vesicles. The data obtained in
the present study indicate that nerolidol acts as an antifungal agent against Candida albicans and Candida
tropicalis, in addition to potentiating (only in the liposomal form) the e ect of fluconazole. However, the compound had little inhibitory e ect on fungal dimorphism. (4) Conclusions: The incorporation
of nerolidol into liposomes improved its antifungal-modulating properties. | pt_BR |
Affilliation | “Múltipla – ver em notas” | pt_BR |
Subject | Nerolidol | pt_BR |
Subject | Liposomes | pt_BR |
Subject | Fluconazole | pt_BR |
Subject | Candida dimorphism | pt_BR |
Subject | Antifungal resistance inhibition | pt_BR |