Author | Dantas, Rebeca C. M. | |
Author | Guimarães, Vanessa Sousa Nazaré | |
Author | Souza, Renata O. de | |
Author | Valverde, Ludmila de Faro | |
Author | Vidal, Manuela T. A. | |
Author | Nogueira, Raphael L. R. | |
Author | Rocha, Leonardo O. S. da | |
Author | Araújo, Giovana T. | |
Author | Santos, Jean N. dos | |
Author | Rocha, Clarissa Araújo Gurgel | |
Access date | 2020-09-10T17:56:48Z | |
Available date | 2020-09-10T17:56:48Z | |
Document date | 2020 | |
Citation | DANTAS, Rebeca C.M. et al. Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma. Applied Immunohistochemistry and Molecular Morphology, 2020. | pt_BR |
ISSN | 1541-2016 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/43303 | |
Language | eng | pt_BR |
Publisher | Lippincott, Williams & Wilkins | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Carcinoma de células escamosas | pt_BR |
Subject in Portuguese | Neoplasias da boca | pt_BR |
Subject in Portuguese | Proteína | pt_BR |
Subject in Portuguese | Transição epitelial-mesenquimal | pt_BR |
Subject in Portuguese | Proliferação celular | pt_BR |
Title | Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma | pt_BR |
Type | Article | pt_BR |
Abstract | In oral squamous cell carcinoma (OSCC), involvement
and activation of the Hedgehog pathway (HH) may be related to
epithelial-mesenchymal transition and cell proliferation. The present
study aimed to evaluate epithelial-mesenchymal transition and
proliferative potential in OSCC cases demonstrating activation of
the HH pathway. Twenty-three GLi-1-positive OSCC cases were
submitted to immunohistochemical detection of Snail, Slug,
N-cadherin, E-cadherin, β-catenin, and MCM3 proteins. Clinicalpathologic
immunoexpression data were obtained from the invasion
front and tumor islets, and then compared. At the invasion
front, OSCC cases presented positive Snail, Slug, and MCM3 expression
in the nuclei of tumor cells. Loss of membrane and cytoplasmic
expression of E-cadherin and β-catenin was also observed.
Positive N-cadherin expression was observed in 31.78% of the cases.
GLi-1 immunoexpression was associated with loss of membrane
E-cadherin (P<0.001), membrane β-catenin (P<0.001), and cytoplasmic
β-catenin (P=0.02) expression. In the tumor islets,
we observed nuclear expression of GLi-1, Snail, Slug, and
MCM3. E-cadherin and β-catenin showed positivity in tumor cell
membranes. Statistically significant positive correlations between
GLi-1 and Snail (P=0.05), E-cadherin (P=0.01), and cytoplasmic
β-catenin (P=0.04) were found. GLi-1 was associated with clinical
staging, while membrane β-catenin expression was related to
the presence of metastasis in lymph nodes and to clinical staging.
The HH pathway may be involved in regulating the expression of
the mesenchymal phenotype. The loss of membrane E-cadherin and
β-catenin expression was observed at the tumor front region,
whereas cell adhesion protein expression was detected in tumor
islets regardless of MCM3. | pt_BR |
Affilliation | Federal University of Bahia. School of Dentistry. Laboratory of Oral Surgical Pathology. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil. | pt_BR |
Subject | Squamous cell carcinoma | pt_BR |
Subject | Mouth neoplasms | pt_BR |
Subject | Hedgehog protein | pt_BR |
Subject | Epithelial-mesenchymal transition, | pt_BR |
Subject | Cell proliferation | pt_BR |