Author | Araujo, Christiane Bezerra de | |
Author | Cunha, Julia Pinheiro Chagas da | |
Author | Inada, Davi Toshio | |
Author | Damasceno, Jeziel | |
Author | Lima, Alex Ranieri Jerônimo | |
Author | Hiraiwa, Priscila | |
Author | Marques, Catarina | |
Author | Gonçalves, Evonnildo | |
Author | Nishiyama-Junior, Milton Yutaka | |
Author | McCulloch, Richard | |
Author | Elias, Maria Carolina | |
Access date | 2020-07-31T19:07:47Z | |
Available date | 2020-07-31T19:07:47Z | |
Document date | 2020 | |
Citation | ARAUJO, Christiane Bezerra de et al. Replication origin location might contribute to genetic variability in Trypanosoma cruzi. BMC Genomics, v. 21, n. 414, p. 1-16, 2020. | pt_BR |
ISSN | 1471-2164 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/42493 | |
Language | por | pt_BR |
Publisher | BMC | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Title | Replication origin location might contribute to genetic variability in Trypanosoma cruzi | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s12864-020-06803-8 | |
Abstract | DNA replication in trypanosomatids operates in a uniquely challenging environment, since most of
their genomes are constitutively transcribed. Trypanosoma cruzi, the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among strains, though the underlying mechanisms are unknown. As results had, we have mapped sites of DNA replication initiation across the T. cruzi genome using Marker Frequency Analysis, which has previously only been deployed in two related trypanosomatids. The putative origins identified in T.cruzi show a notable enrichment of GC content, a preferential position at subtelomeric regions, coinciding with genes transcribed towards the telomeres, and a pronounced enrichment within coding DNA sequences, most notably in genes from the Dispersed Gene Family 1 (DGF-1). Therefore as conclusions, these findings suggest a scenario where collisions between DNA replication and transcription are frequent, leading to increased genetic variability, as seen by the increase SNP levels at chromosome subtelomeres and in DGF-1 genes containing putative origins. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil. | pt_BR |
Affilliation | The Wellcome Centre for Molecular Parasitology. Institute of Infection, Immunity and Inflammation. University of Glasgow. Glasgow, UK. | pt_BR |
Affilliation | Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular – Bioinformática. Belém, PA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | The Wellcome Centre for Molecular Parasitology. Institute of Infection, Immunity and Inflammation. University of Glasgow. Glasgow, UK. | pt_BR |
Affilliation | Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Tecnologia Biomolecular – Bioinformática. Belém, PA, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil / Instituto Butantan. Laboratório Especial de Toxinologia Aplicada. Butantã, SP, Brasil. | pt_BR |
Affilliation | The Wellcome Centre for Molecular Parasitology. Institute of Infection, Immunity and Inflammation. University of Glasgow. Glasgow, UK. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. Butantã, SP, Brasil / Instituto Butantan. Centro de Toxinas, Resposta-imune e Sinalização Celular. Butantã, SP, Brasil. | pt_BR |
Subject | Replication Origin | pt_BR |
Subject | Genetic Variation | pt_BR |
Subject | Interspersed Repetitive Sequences | pt_BR |
Subject in Spanish | Origen de Réplica | pt_BR |
Subject in Spanish | Variación Genética | pt_BR |
Subject in Spanish | Secuencias Repetitivas Esparcidas | pt_BR |
DeCS | Origem de Replicação | pt_BR |
DeCS | Variação Genética | pt_BR |
DeCS | Sequências Repetitivas Dispersas | pt_BR |
e-ISSN | 1471-2164 | |