Author | Silva, Isabelle Leticia Zaboroski | |
Author | Robert, Anny Waloski | |
Author | Cabo, Guillermo Cabrera | |
Author | Spangenberg, Lucia | |
Author | Stimamiglio, Marco Augusto | |
Author | Dallagiovanna, Bruno | |
Author | Gradia, Daniela Fiori | |
Author | Shigunov, Patrícia | |
Access date | 2020-07-01T18:12:33Z | |
Available date | 2020-07-01T18:12:33Z | |
Document date | 2020 | |
Citation | SILVA, Isabelle Leticia Zaboroski et al. Effects of PUMILIO1 and PUMILIO2 knockdown on cardiomyogenic differentiation of human embryonic stem cells culture. PLoS ONE., v. 15, n. 5, p. 1-18, 2020. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/42031 | |
Language | por | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Pumilio | pt_BR |
Title | Effects of PUMILIO1 and PUMILIO2 knockdown on cardiomyogenic differentiation of human embryonic stem cells culture | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0222373 | |
Abstract | Posttranscriptional regulation plays a fundamental role in the biology of embryonic stem cells (ESCs). Many studies have demonstrated that multiple mRNAs are coregulated by one or more RNA-binding proteins (RBPs) that orchestrate mRNA expression. A family of RBPs, which is known as the Pumilio-FBF (PUF) family, is highly conserved among different species and has been associated with the undifferentiated and differentiated states of different cell lines. In humans, two homologs of the PUF family have been found: Pumilio 1 (PUM1) and Pumilio 2 (PUM2). To understand the role of these proteins in human ESCs (hESCs), we first assessed the influence of the silencing of PUM1 and PUM2 on pluripotency genes and found that the knockdown of Pumilio genes significantly decreased the OCT4 and NANOG mRNA levels and reduced the amount of nuclear OCT4, which suggests that Pumilio proteins play a role in the maintenance of pluripotency in hESCs. Furthermore, we observed that PUM1-and-PUM2-silenced hESCs exhibited improved efficiency of in vitro cardiomyogenic differentiation. Through an in silico analysis, we identified mRNA targets of PUM1 and PUM2 that are expressed at the early stages of cardiomyogenesis, and further investigation will determine whether these target mRNAs are active and involved in the progression of cardiomyogenesis. Our findings contribute to the understanding of the role of Pumilio proteins in hESC maintenance and differentiation. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Bioinformatics Unit. Instituto Pasteur de Montevideo. Montevideo, Uruguay. | pt_BR |
Affilliation | Bioinformatics Unit. Instituto Pasteur de Montevideo. Montevideo, Uruguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Subject | Embryonic Stem Cells | pt_BR |
Subject | RNA Processing, Post-Transcriptional | pt_BR |
Subject | Cell Differentiation | pt_BR |
Subject in Spanish | Células Madre Embrionarias | pt_BR |
Subject in Spanish | Procesamiento Postranscripcional del ARN | pt_BR |
Subject in Spanish | Diferenciación Celular | pt_BR |
DeCS | Células-Tronco Embrionárias | pt_BR |
DeCS | Processamento Pós-Transcricional do RNA | pt_BR |
DeCS | Diferenciação Celular | pt_BR |
e-ISSN | 1932-6203 | |