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A LINEAGE-SPECIFIC RAPID DIAGNOSTIC TEST (CHAGAS SERO K-SET) IDENTIFIES BRAZILIAN TRYPANOSOMA CRUZI II/V/VI RESERVOIR HOSTS AMONG DIVERSE MAMMALIAN ORDERS
Teste de Diagnóstico
Hospedeiros
Mamíferos
Chagas Sero K-Set
Trypanosma cruzi
Author
Affilliation
London School of Hygiene and Tropical Medicine. Faculty of Infectious Diseases. London, United Kingdom.
London School of Hygiene and Tropical Medicine. Faculty of Infectious Diseases. London, United Kingdom.
Coris BioConcept. Gembioux, Belgium.
London School of Hygiene and Tropical Diseases. London, United Kingdom.
Coris Bioconcept. Gembioux, Belgium.
Coris Bioconcept. Gemboioux, Belgium.
Coris Bioconcept. Gemboioux, Belgium.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
London School of Hygiene and Tropical Medicine. Faculty of Infectious and Tropical Diseases. London, United Kingdom.
London School of Hygiene and Tropical Medicine. Faculty of Infectious Diseases. London, United Kingdom.
Coris BioConcept. Gembioux, Belgium.
London School of Hygiene and Tropical Diseases. London, United Kingdom.
Coris Bioconcept. Gembioux, Belgium.
Coris Bioconcept. Gemboioux, Belgium.
Coris Bioconcept. Gemboioux, Belgium.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
London School of Hygiene and Tropical Medicine. Faculty of Infectious and Tropical Diseases. London, United Kingdom.
Abstract
Trypanosoma cruzi, the protozoan agent of Chagas disease in the Americas, is comprised
of six genetic lineages (TcI-TcVI) and a possible seventh (TcBat, related to TcI). Identification
of T. cruzi lineages infecting reservoir mammalian species is fundamental to resolving
transmission cycles. However, this is hindered by the limited sensitivity and technical complexity
of parasite isolation and genotyping. An alternative approach is serology using T.
cruzi lineage-specific epitopes, such as those of the trypomastigote small surface antigen
(TSSA). For surveillance of T. cruzi lineage infections in mammal species from diverse Brazilian
regions, we apply a novel rapid diagnostic test (RDT, Chagas Sero K-SeT), which
incorporates the TSSA peptide epitope specific to TcII/V/VI (TSSApep-II/V/VI) and Protein
G detection of antibodies. Chagas Sero K-SeT RDT results with sera from experimentally
infected mice, from tamarin primates (Leontopithecus spp.) and from canines (Canis familiaris)
were concordant with corresponding TSSApep-II/V/VI ELISAs. The Chagas Sero KSet
detected TcII/V/VI infections in Leontopithecus spp. from the Atlantic forest (n = 46), in
C. familiaris (n = 16) and Thrichomys laurentius (n = 2) from Caatinga biome and Chiroptera
(n = 1) from Acre, Amazonia. The Chagas Sero K-SeT RDT is directly applicable to TcII/V/
VI-specific serological surveillance of T. cruzi infection in several different mammalian
Orders. It can replace ELISAs and provides efficient, point-of-sampling, low-cost detection
of TcII/V/VI infections, with at least equivalent sensitivity, although some mammals may be
difficult to trap, and, not unexpectedly, Chagas Sero K-SeT could not recognise feline IgG.
Knowledge of sylvatic hosts of T. cruzi can be expanded, new reservoir species discovered,
and the ecology of transmission cycles clarified, particularly with adaptation to further mammalian orders.
Keywords in Portuguese
Trypanosma cruziTeste de Diagnóstico
Hospedeiros
Mamíferos
Chagas Sero K-Set
Trypanosma cruzi
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