Author | Ribeiro, Christiana Vargas | |
Author | Rocha, Bruna Fonte Boa | |
Author | Oliveira, Edward José de | |
Author | Carvalho, Andrea Teixeira de | |
Author | Martins Filho, Olindo Assis | |
Author | Murta, Silvane Maria Fonseca | |
Author | Pascoal, Vanessa Peruhype Magalhães | |
Access date | 2020-05-19T14:02:30Z | |
Available date | 2020-05-19T14:02:30Z | |
Document date | 2020 | |
Citation | RIBEIRO, Christiana Vargas et al. Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte. Mem. Inst. Oswaldo Cruz, v. 115, e190408, 2020 | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/41301 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | L. braziliensis | pt_BR |
Subject in Portuguese | L. infantum | pt_BR |
Title | Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/0074-02760190408 | |
Abstract | BACKGROUND: The mechanism of resistance to SbIII in Leishmania is complex, multifactorial and involves not only biochemical mechanisms, but also other elements, such as the immune system of the host.
OBJECTIVES: In this study, putative changes in the immunological profile of human monocytes infected with wild-type (WT) and antimony (SbIII)-resistant Leishmania (Viannia) braziliensis and Leishmania (Leishmania) infantum lines were evaluated.
METHODS: Susceptibility assays WT and SbIII-resistant L. braziliensis and L. infantum were performed using lines THP-1 human monocytic lineage. Phagocytic capacity, cytokine profile, intracellular nitric oxide (NO) production and surface carbohydrate residues profile were performed in peripheral blood monocytes by flow cytometry.
FINDINGS: The phagocytic capacity and intracellular NO production by classical (CD14++CD16-) and proinflammatory (CD14++CD16+) monocytes were higher in the presence of L. infantum lines compared to L. braziliensis lines. The results also highlight proinflammatory monocytes as the cellular subpopulation of major relevance in a phagocytosis event and NO expression. It is important to note that L. infantum induced a proinflammatory cytokine profile characterised by higher levels of TNF-α in culture supernatant than L. braziliensis. Conversely, both Leishmania lines induce high levels of IL-6 in culture supernatant. Analysis of the expression profile of surface carbohydrates showed that L. braziliensis presents 4.3-fold higher expression of galactose(β1,4)N-acetylglucosamine than L. infantum line. Interestingly, the expression level of α-N-acetylgalactosamine residues was 2-fold lower in the SbIII-resistant L. braziliensis line than its counterpart WT line, indicating differences in surface glycoconjugates between these lines.
MAIN CONCLUSIONS: Our results showed that L. braziliensis and L. infantum induce different innate immune responses and a highly inflammatory profile, which is characteristic of infection by L. infantum, the species associated with visceral disease. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Genômica Funcional e Proteômica de Leishmania spp. e Trypanosoma cruzi. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Genômica Funcional e Proteômica de Leishmania spp. e Trypanosoma cruzi. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Genômica Funcional e Proteômica de Leishmania spp. e Trypanosoma cruzi. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Genômica Funcional e Proteômica de Leishmania spp. e Trypanosoma cruzi. Belo Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil | pt_BR |
Subject | L. braziliensis | pt_BR |
Subject | L. infantum | pt_BR |
Subject | monocyte subsets | pt_BR |
Subject | lectins | pt_BR |
Subject | cytokines | pt_BR |
Subject | nitric oxide | pt_BR |
Subject | surface glycoconjugates | pt_BR |