Author | Melo, Christiane Bandeira | |
Author | Paiva, Ligia Almeida | |
Author | Amorim, Natália R. T. | |
Author | Weller, Peter F. | |
Author | Bozza, Patrícia T. | |
Access date | 2020-04-20T15:05:00Z | |
Available date | 2020-04-20T15:05:00Z | |
Document date | 2017 | |
Citation | MELO, Christianne Bandeira et al. EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis. Methods in Molecular Biology, v. 1554, p. 127-141, 2017. | pt_BR |
ISSN | 1064-3745 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/40886 | |
Description | Acesso aberto em 20/04/2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774667/ | pt_BR |
Language | eng | pt_BR |
Publisher | Humana Press | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Biossíntese | pt_BR |
Subject in Portuguese | Carbodiimida | pt_BR |
Subject in Portuguese | Compartimentalização | pt_BR |
Subject in Portuguese | Eicosanóides | pt_BR |
Subject in Portuguese | Leucotrieno | pt_BR |
Subject in Portuguese | Gotículas lipídicas | pt_BR |
Subject in Portuguese | Perinuclear | pt_BR |
Subject in Portuguese | Fagossomos | pt_BR |
Subject in Portuguese | Prostaglandina | pt_BR |
Title | EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1007/978-1-4939-6759-9_6 | |
Abstract | Eicosanoids are bioactive lipids derived from enzymatic metabolism of arachidonic acid via the cyclooxygenase (COX) and lipoxygenase (LOX) pathways. These lipids are newly formed and nonstorable molecules that have important roles in physiological and pathological processes. The particular interest to determine intracellular compartmentalization of eicosanoid-synthetic machinery has emerged as a key component in the regulation of eicosanoid synthesis and in delineating functional intracellular and extracellular actions of eicosanoids. In this chapter, we discuss the EicosaCell protocol, an assay that enables the intracellular detection and localization of eicosanoid lipid mediator-synthesizing compartments by means of a strategy to covalently cross-link and immobilize eicosanoids at their sites of synthesis followed by immunofluorescent-based localization of the targeted eicosanoid. EicosaCell assays have been successfully used to identify different intracellular compartments of synthesis of prostaglandins and leukotrienes upon cellular activation. This chapter covers basics of EicosaCell assay including its selection of reagents, immunodetection design as well as some troubleshooting recommendations. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Harvard Medical Scholo. Department of Medicine. Beth Israel Deaconess Medical Center. Boston, MA, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Biosynthesis | pt_BR |
Subject | Carbodiimide | pt_BR |
Subject | Compartmentalization | pt_BR |
Subject | EDAC | pt_BR |
Subject | Eicosanoids; | pt_BR |
Subject | Leukotriene | pt_BR |
Subject | Lipid droplets | pt_BR |
Subject | Perinuclear | pt_BR |
Subject | Phagosomes | pt_BR |
Subject | Prostaglandin | pt_BR |
e-ISSN | 1940-6029 | |