Author | Crispim, Myuki Alfaia Esashika | |
Author | Reis, Mônica Nogueira da Guarda | |
Author | Abrahim, Cláudia Maria de Moura | |
Author | Kiesslich, Dagmar | |
Author | Fraiji, Nelson | |
Author | Bello, Gonzalo | |
Author | Stefani, Mariane Martins Araújo | |
Access date | 2020-04-16T13:54:51Z | |
Available date | 2020-04-16T13:54:51Z | |
Document date | 2019 | |
Citation | CRISPIM, Myuki Alfaia Esashika et al. Homogenous HIV-1 subtype B from the Brazilian Amazon with infrequent diverse BF1 recombinants, subtypes F1 and C among blood donors. Plos One, v. 14, n. 9, e0221151, 16p, Sept. 2019. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/40801 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | HIV-1 homogêneo, subtipo B | pt_BR |
Subject in Portuguese | Recombinantes subtipos F1 e C | pt_BR |
Subject in Portuguese | Doadores de sangue | pt_BR |
Subject in Portuguese | Amazônia brasileira | pt_BR |
Title | Homogenous HIV-1 subtype B from the Brazilian Amazon with infrequent diverse BF1 recombinants, subtypes F1 and C among blood donors | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pone.0221151 | |
Abstract | In the last decade a growing HIV/AIDS epidemic with increased incidence and AIDS-related mortality has been reported in Northern Brazil from which molecular data are scarce. Also, apparently healthy, adult blood donors, recently diagnosed with HIV-1 represent important sentinel populations for molecular studies. This cross-sectional study describes HIV-1 subtypes in blood donors from three reference public blood centers located in three States in Northern Brazil. HIV-1 pol sequencing (protease/PR, reverse transcriptase/RT) was performed on plasma samples of HIV-1 positive donors from HEMOAM, Manaus, Amazonas (n = 198), HEMERON, Porto Velho, Rondônia (n = 20) and HEMORAIMA, Boa Vista, Roraima (n = 9) collected from 2011-2017. HIV-1 subtypes were identified by REGA, phylogenetic inference; recombinant viruses were characterized by SIMPLOT. Young, single, males predominated, around half was first-time donors. Syphilis co-infection was detected in 17% (39 out of 227), 8% (18 out of 227) was anti-HBc positive. Subtype B represented ≥ 90% in Amazonas, Rondônia and Roraima, subtype C (3.1%) was found in Amazonas and Rondônia; subtype F1 (0.9%) and BF1 recombinants (5.3%) were only detected in Amazonas. Subtype B sequences from Amazonas (n = 179), Rondônia (n = 18) and Roraima (n = 9) were combined with viral strains representative of the BPANDEMIC (n = 300) and BCARIBBEAN/BCAR (n = 200) lineages. The BPANDEMIC lineage predominated (78%) although BCAR lineages were frequent in Roraima (56%) and Amazonas (22%). Subtype C and subtype F1 sequences identified here clustered within Brazilian CBR and F1BR lineages, respectively. Twelve BF1 mosaics showed 11 different recombination profiles: six were singleton unique-recombinant-forms/URFs, one displays a CRF28/29_BF-like recombinant pattern and the remaining four BF1 isolates branched with other Brazilian BF1 viruses previously described and may represent putative new CRF_BF1 from Northern Brazil. Our study shows a highly homogeneous molecular pattern with prevalent subtype B, followed by BF1, and sporadic subtype C and F1 in blood donors from the Northern region. Surveillance studies are important to monitor HIV-1 diversity which can reveal patterns of viral dissemination, especially in a highly endemic, remote and geographically isolated region as Northern Brazil. | pt_BR |
Affilliation | Fundação de Hematologia e Hemoterapia do Amazonas/HEMOAM. Manaus, AM, Brasil. | pt_BR |
Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da AIDS e da Hanseniase. Goiânia, GO, Brasil. | pt_BR |
Affilliation | Fundação de Hematologia e Hemoterapia do Amazonas/HEMOAM. Manaus, AM, Brasil. | pt_BR |
Affilliation | Fundação de Hematologia e Hemoterapia do Amazonas/HEMOAM. Manaus, AM, Brasil. | pt_BR |
Affilliation | Fundação de Hematologia e Hemoterapia do Amazonas/HEMOAM. Manaus, AM, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da AIDS e da Hanseniase. Goiânia, GO, Brasil. | pt_BR |
Subject | HIV-1 subtype B | pt_BR |
Subject | Brazilian Amazon | pt_BR |
Subject | Subtypes F1 and C | pt_BR |
Subject | Blood donors | pt_BR |