Author | Damasio, Marcos Paulo de Souza | |
Author | Rocha, Manoel Otávio da Costa | |
Author | Sousa, Giovane Rodrigo | |
Author | Ferreira, Karine Silvestre | |
Author | Gusmão, Rafaelle Cristina Gomes Fares | |
Author | Medeiros, Nayara Ingrid | |
Author | Araujo, Fernanda Fortes de | |
Author | Chaves, Ana Thereza | |
Author | Dutra, Walderez Ornelas | |
Author | Oliveira, Rodrigo Corrêa de | |
Author | Estanislau, Juliana de Assis Silva Gomes | |
Access date | 2020-02-18T16:13:22Z | |
Available date | 2020-02-18T16:13:22Z | |
Document date | 2019 | |
Citation | DAMASIO, Marcos Paulo de Souza et al. PD1 and PDL1 molecules control suppressor activity of regulatory T cells in chronic Chagas cardiomyopathy patients. Human Immunology, v. 80, n. 7, p. 517-522, 2019. | pt_BR |
ISSN | 0198-8859 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/40049 | |
Language | eng | pt_BR |
Publisher | Elsevier/North-Holland | pt_BR |
Rights | restricted access | pt_BR |
Title | PD1 and PDL1 molecules control suppressor activity of regulatory T cells in chronic Chagas cardiomyopathy patients | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.humimm.2019.03.009 | |
Abstract | Introduction: Chagas disease, caused by the protozoan Trypanosoma cruzi (T. cruzi), is the fourth most important tropical disease, which affects approximately 7 million people worldwide. The mechanisms involved in the development of this disease are not completely well understood. An important protective role of regulatory T cells (Treg) in Chagas disease has been observed; however, the specific mechanisms remain unclear. We evaluated apoptosis as a possible mechanism mediated by Treg cells (CD4+CD25HighFOXP3+) to orchestrate the immune response in chronic Chagas disease.
Methods and results: Patients with Chagas disease were grouped as the indeterminate (IND; asymptomatic patients with Chagas disease; n = 10) and dilated cardiomyopathy (CARD; n = 10). Healthy T. cruzi-negative individuals (NI; n = 10) were included as a control group. In order to evaluate the apoptotic cell profile, the expression of PD1, PD1L, CD39, CD95, CD95L molecules were investigated. We also evaluated the proportion of CD14+ cells expressing caspase 3. The IND group presented a substantially higher expression of CD39 by Treg cells as compared to the CARD group. On the other hand, the CARD group showed higher expression of PD-1 by Treg cells than both NI and IND groups. Significant positive correlations were observed between Treg CD95L+ cells and CD14 cells expressing caspase 3 as well as between Treg CD39 cells and CD14+ Caspase3+ cells in the IND group.
Conclusion: Our data indicate that the expressions of different molecules that induce apoptosis are associated with suppressive mechanisms mediated by Treg cells and suggest a possible role for PD1 and PDL1 molecules in the morbidity of chronic Chagas disease. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade de Uberaba. Medicina Veterinária. Mestrado em Sanidade e Produção Animal nos Trópicos, Uberaba, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisa em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brasil. | pt_BR |
Subject | Chagas disease | pt_BR |
Subject | Regulatory T cells | pt_BR |
Subject | Apoptotic mechanisms | pt_BR |
Embargo date | 2100-01-01 | |