Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/39139
Type
ArticleCopyright
Open access
Embargo date
2021-01-10
Collections
- INI - Artigos de Periódicos [3398]
Metadata
Show full item record
PREVALENCE OF CANDIDA DUBLINIENSIS IN THE BCCM/IHEM BIOMEDICAL FUNGI/YEASTS CULTURE COLLECTION (ISOLATES BEFORE 1990)
Affilliation
Scientific Institute of Public Health. Brussels, Belgium / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Scientific Institute of Public Health. Brussels, Belgium / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Scientific Institute of Public Health. Brussels, Belgium.
Scientific Institute of Public Health. Brussels, Belgium / Institute of Tropical Medicine. Antwerp, Belgium.
Scientific Institute of Public Health. Brussels, Belgium.
Scientific Institute of Public Health. Brussels, Belgium / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Scientific Institute of Public Health. Brussels, Belgium.
Scientific Institute of Public Health. Brussels, Belgium / Institute of Tropical Medicine. Antwerp, Belgium.
Scientific Institute of Public Health. Brussels, Belgium.
Abstract
The BCCM/IHEM Biomedical Fungi/Yeasts collection hosts 1200 Candida albicans strains of the Vanbreuseghem mycotheque isolated between 1951 and 1997. From this collection, 469 freeze-dried C. albicans strains, producing chlamydospores, germ tubes and forming green colonies on CHROMagar, all isolated before 1990, were screened to identify the Candida dubliniensis isolates. Screening was performed in different steps using the growth at 45 degrees C, the assimilation of xylose, the intracellular beta-glucosidase activity test and C. dubliniensis-specific polymerase chain reaction (PCR) with primers from ACT1 intron sequence. Five isolates (1%) were identified as C. dubliniensis: one isolate was not documented, the others were of oropharyngeal origin of which two (1987 and 1990) were from proven human immunodeficiency virus patients.
Share