Author | Amado, Luciane A. | |
Author | Villar, Livia M. | |
Author | Paula, Vanessa S. de | |
Author | Almeida, Adilson J. de | |
Author | Gaspar, Ana Maria C. | |
Access date | 2019-12-19T16:49:50Z | |
Available date | 2019-12-19T16:49:50Z | |
Document date | 2006 | |
Citation | AMADO, Luciana A. et al. Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 2, p. 149-165, Mar. 2006. | pt_BR |
ISSN | 0074-0206 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/38720 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Fluido oral | pt_BR |
Subject in Portuguese | Hepatites virais | pt_BR |
Subject in Portuguese | Diagnóstico | pt_BR |
Subject in Portuguese | Epidemiologia | pt_BR |
Title | Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/s0074-02762006000200006 | |
Abstract | In this report, we examine the adaptability of commercially available serological kits to detect antibodies markers for viral hepatitis in oral fluid samples. We also assessed the prevalence of hepatitis A, B, and C virus-specific antibodies, and related risk factors for these infectious diseases through sensitivity of the tests in saliva samples to evaluate if oralfluid can be an alternative tool to substitute serum in diagnosis of acute viral hepatitis and in epidemiological studies. One hundred and ten paired serum and saliva specimens from suspect patients of having acute hepatitis were collected to detect antibodies to hepatitis A (total and IgM), hepatitis B (anti-HBs, total anti-HBc and IgM anti-HBc), and hepatitis C (anti-HCV) using commercially available enzyme-linked immunossorbent assay (EIA). In relation to serum samples, oral fluid assay sensitivity and specificity were as follows: 87 and 100% for total anti-HAV, 79 and 100% for anti-HAVIgM, 6 and 95% for anti-HBs, 13 and 100%for total anti-HBc, 100 and 100% for anti-HBc IgM, and 75 and 100% for anti-HCV The consistency observed between antibodies tests in saliva and expected risk factors for hepatitis A and C suggests that the saliva method could replace serum in epidemiological studies for hepatitis A and C. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Oral fluid | pt_BR |
Subject | Viral hepatitis | pt_BR |
Subject | Diagnostic and epidemiology | pt_BR |
e-ISSN | 1678-8060 | |