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https://www.arca.fiocruz.br/handle/icict/38645
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ArticleCopyright
Open access
Embargo date
2020-06-17
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- INI - Artigos de Periódicos [3393]
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IMMUNIZATION WITH RECOMBINANT ENOLASE OF SPOROTHRIX SPP. (RSSENO) CONFERS EFFECTIVE PROTECTION AGAINST SPOROTRICHOSIS IN MICE
Author
Portuondo, Deivys Leandro
Dores-Silva, Paulo Roberto
Ferreira, Lucas Souza
Oliveira, Carlos S. de
Téllez-Martínez, Damiana
Marcos, Caroline Maria
Loesch, Maria Luiza de Aguiar
Guzmán, Fanny
Gava, Lisandra M.
Borges, Júlio César
Pereira, Sandro Antonio
Batista-Duharte, Alexander
Carlos, Iracilda Zeppone
Dores-Silva, Paulo Roberto
Ferreira, Lucas Souza
Oliveira, Carlos S. de
Téllez-Martínez, Damiana
Marcos, Caroline Maria
Loesch, Maria Luiza de Aguiar
Guzmán, Fanny
Gava, Lisandra M.
Borges, Júlio César
Pereira, Sandro Antonio
Batista-Duharte, Alexander
Carlos, Iracilda Zeppone
Affilliation
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
University of São Paulo. São Carlos Institute of Chemistry. São Carlos, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
University of São Paulo. São Carlos Institute of Chemistry. São Carlos, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
Pontificia Universidad Católica de Valparaíso. Núcleo Biotecnológico de Curauma. Valparaíso, Chile.
Federal University of São Carlos. Center of Biological and Health Sciences. São Carlos, SP, Brazil.
University of São Paulo. São Carlos Institute of Chemistry. São Carlos, SP, Brazil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
University of São Paulo. São Carlos Institute of Chemistry. São Carlos, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
University of São Paulo. São Carlos Institute of Chemistry. São Carlos, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
Pontificia Universidad Católica de Valparaíso. Núcleo Biotecnológico de Curauma. Valparaíso, Chile.
Federal University of São Carlos. Center of Biological and Health Sciences. São Carlos, SP, Brazil.
University of São Paulo. São Carlos Institute of Chemistry. São Carlos, SP, Brazil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
São Paulo State University. School of Pharmaceutical Sciences. Department of Clinical Analysis. Araraquara, SP, Brazil.
Abstract
In recent years, research has focused on the immunoreactive components of the Sporothrix schenckii cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of S. schenckii cell wall proteins. Here, we sought to assess the protective potential of a Sporothrix spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the S. brasiliensis infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with S. brasiliensis as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after in vitro stimulation of splenic cells with rSsEno: elevated levels of IFN-γ and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both S. schenckii and S. brasiliensis. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis.
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