Author | Neves, Renata Heisler | |
Author | Alencar, Alba Cristina Miranda de Barros | |
Author | Aguila, Marcia Barbosa | |
Author | Lacerda, Carlos Alberto Mandarim de | |
Author | Silva, José Roberto Machado | |
Author | Gomes, Delir Corrêa | |
Access date | 2019-10-22T15:52:55Z | |
Available date | 2019-10-22T15:52:55Z | |
Document date | 2006 | |
Citation | NEVES, Renata Heisler et al. Hepatic stereology of schistosomiasis mansoni infected-mice fed a high-fat diet. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, Suppl. I, p. 253-260, 2006. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/36649 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Schistosoma mansoni | pt_BR |
Subject in Portuguese | Estereologia | pt_BR |
Subject in Portuguese | Fígado | pt_BR |
Subject in Portuguese | Dieta rica em gordura | pt_BR |
Subject in Portuguese | Ratos | pt_BR |
Title | Hepatic stereology of Schistosomiasis mansoni infected-mice fed a high-fat diet | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/s0074-02762006000900039 | |
Abstract | High-fat diets induce weight gain and fatty liver in wild-type mice. Schistosomiasis mansoni infection also promotes hepatic injury. This study was designed to quantify hepatic alterations in schistosomiasis mansoni-infected mice fed a high fat-rich chow compared to mice fed a standard rodent chow, using stereology. Female SW mice fed each either high-fat diet (29% lipids) or standard chow (12% lipids) over 8 months, and then were infected with Schistosoma mansoni cercariae. Four experimental groups were studied: infected mice fed a high-fat diet (IHFC) or standard chow (ISC), uninfected mice fed a high-fat diet (HFC) or standard chow (SC). Mice were sacrificed during early infection (9 weeks from exposure). The following hepatic biometry and the stereology parameters were determined: volume density (hepatocytes [h], sinusoids [s], steatosis [st] and hepatic fibrosis [hf]); numerical density (hepatocyte nuclei - Nv[h]); absolute number of total hepatocyte N[h], normal hepatocyte N[nh], and binucleated hepatocyte N[bh], percentage of normal hepatocyte P[nh] and binucleated hepatocyte P[bh]. IHFC and HFC groups exhibited TC, HDL-C, LDL-C, and body mass significantly greater (p < 0.05) than control group. No significant differences were found regards liver volume (p = 0.07). Significant differences were observed regards P[nh] (p = 0.0045), P[bh] (p = 0.0045), Nv[h] (p = 0.0006), N[h] (p = 0.0125), N[bh] (p = 0.0164) and N[nh] (p = 0.0078). IHFC mice group presented 29% of binucleated hepatocytes compared to HFC group (19%), ISC group (17%) and SC (6%). Volume density was significantly different between groups: Vv[h] (p = 0.0052), Vv[s] (p = 0.0025), Vv[st] (p = 0.0004), and Vv[hf] (p = 0.0007). In conclusion, schistosomiasis mansoni infection with concurrent high-fat diet promotes intensive quantitative changes in hepatic structure, contributing to an increasing on hepatic regeneration. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Patologia e Laboratórios. Laboratório de Helmintologia Romero Lascasas Porto. Disciplina de Parasitologia. Ri de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Laboratório de Morfometria e Morfologia Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Laboratório de Morfometria e Morfologia Cardiovascular. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Patologia e Laboratórios. Laboratório de Helmintologia Romero Lascasas Porto. Disciplina de Parasitologia. Ri de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Helmintos Parasitos de Vertebrados. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Schistosoma mansoni | pt_BR |
Subject | Stereology | pt_BR |
Subject | Liver | pt_BR |
Subject | High-fat diet | pt_BR |
Subject | Mice | pt_BR |
e-ISSN | 1678-8060 | |