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2022-01-01
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TRYPANOSOMA RANGELI: EFFECTS OF PHYSALIN B ON THE IMMUNE REACTIONS OF THE INFECTED LARVAE OF RHODNIUS PROLIXUS
Seco-steroids
Physalis angulata L
Physalin
Trypanosoma rangeli
Hemocytes
Microaggregation
Nitric Oxide
Prophenoloxidase
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract
Physalins are seco-steroids obtained from plants of the family Solanaceae. Herein, we tested Physalis angulata L puriWed physalin B as
an immunomodulatory compound in 5th-instar larvae of Rhodnius prolixus, which were systemically infected with the H14 Trypanosoma
rangeli strain protozoan. In uninfected insects, the eVective concentration of physalin B, which inhibited 50% of the blood ingested (ED50)
volume, was 15.2§1.6 g/ml of the meal. Ecdysis processes and mortality in uninfected larvae, treated orally with physalin B in concentrations
ranging from 1 to 10 g/ml, was similar to that observed in insects not treated with physalin B. However, R. prolixus larvae previously
fed on blood containing 1.0, 0.1, and 0.01 g of physalin B/ml exhibited mortality rates of 78.1, 54.3, and 12.7%, respectively, 6 days
after inoculation of T. rangeli (1£103 parasites/insect), whereas only 7.2% mortality was observed in the control group, injected with sterile
culture medium. The insects treated with physalin B (0.1 g/ml) and inoculated with T. rangeli did not modify the phenoloxidase (PO)
activity and total hemocyte count in the hemolymph. However, physalin B treatment caused a reduction in hemocyte microaggregation
and nitric oxide production and enhanced the parasitemia in the hemolymph. These results demonstrate that physalin B from P. angulata
is a potent immunomodulatory substance for the bloodsucking insect, R. prolixus.
Keywords
Rhodnius prolixusSeco-steroids
Physalis angulata L
Physalin
Trypanosoma rangeli
Hemocytes
Microaggregation
Nitric Oxide
Prophenoloxidase
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