Author | Araujo-Jorge, Tania C. | |
Author | Barbosa, Helene S. | |
Author | Meirelles, Maria Nazareth L. | |
Access date | 2019-10-02T14:25:52Z | |
Available date | 2019-10-02T14:25:52Z | |
Document date | 1992 | |
Citation | ARAUJO-JORGE, Tania C. de; BARBOSA, Helene S.; MEIRELLES, Maria Nazareth L. Trypanosoma cruzi recognition by macrophages and muscle cells: perspectives after a 15-year study. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, Suppl. V, p. 43-56, 1992. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/36083 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Macrófagos | pt_BR |
Subject in Portuguese | Ultraestrutura | pt_BR |
Subject in Portuguese | Miócitos Cardíacos | pt_BR |
Subject in Portuguese | Lectinas | pt_BR |
Subject in Portuguese | Endocitose | pt_BR |
Subject in Portuguese | Proteases | pt_BR |
Title | Trypanosoma cruzi recognition by macrophages and muscle cells: perspectives after a 15-year study | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02761992000900006 | |
Abstract | Macrophages and muscle cells are the main targets for invasion of Trypanosoma cruzi. Ultrastructural studies of this phenomenon in vitro showed that invasion occurs by endocytosis, with attachment and internalization being mediated by different components capable of recognizing epi-or trypomastigotes (TRY). A parasitophorus vacuole was formed in both cell types, thereafter fusing with lysosomes. Then, the mechanism of T. cruzi invasion of host cells (HC) is essentially similar (during a primary infection in the abscence of a specific immune response), regardless of wether the target cell is a professional or a non-professional phagocytic cell. Using sugars, lectins, glycosidases, proteinases and proteinase inhibitors, we observed that the relative balance between exposed sialic acid and galactose/N-acetyl galactosamine (GAL) residues on the TRY surface, determines the parasite's capacity to invade HC, and that lectin-mediated phagocytosis with GAL specificity is important for internalization of T. cruzi into macrophages. On the other hand, GAL on the surface to heart muscle cells participate on TRY adhesion. TRY need to process proteolytically both the HC and their own surface, to expose the necessary ligands and receptors that allow binding to, and internalization in the host cell. The diverse range of molecular mechanisms which the parasite could use to invade the host cell may correspond to differences in the available "receptors"on the surface of each specific cell type. Acute phase components, with lectin or proteinase inhibitory activities (a-macroglobulins), may also be involved in T. cruzi-host cell interaction. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Macrophage | pt_BR |
Subject | Heart muscle cell | pt_BR |
Subject | Lectins | pt_BR |
Subject | Ligand-receptor interaction | pt_BR |
Subject | Ultrastructure | pt_BR |
Subject | Endocytosis | pt_BR |
Subject | Proteinases | pt_BR |
Subject | Alpha-2-macroglobulin | pt_BR |
DeCS | Miócitos Cardíacos | pt_BR |
DeCS | alfa-Macroglobulinas | pt_BR |
e-ISSN | 1678-8060 | |