Author | Cruz, Derciliano Lopes da | |
Author | Paiva, Marcelo Henrique Santos | |
Author | Guedes, Duschinka Ribeiro Duarte | |
Author | Alves, Joana | |
Author | Gómez, Lara Ferrero | |
Author | Ayres, Constância Flávia Junqueira | |
Access date | 2019-09-24T15:13:29Z | |
Available date | 2019-09-24T15:13:29Z | |
Document date | 2019 | |
Citation | CRUZ, Derciliano Lopes da et al. Detection of alleles associated with resistance to chemical insecticide in the malaria vector Anopheles arabiensis in Santiago, Cabo Verde. Malaria Journal, v. 18, n. 1, p. 111, Apr. 2019. | pt_BR |
ISSN | 1475-2875 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/35852 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Detection of alleles associated with resistance to chemical insecticide in the malaria vector Anopheles arabiensis in Santiago, Cabo Verde | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s12936-019-2757-3 | |
Abstract | BACKGROUND:
Mosquitoes of the Anopheles gambiae complex are the main malaria vectors worldwide. Due to the lack of a vaccine to prevent malaria, the principal way to reduce the impact of this disease relies on the use of chemical insecticides to control its vectors. However, the intensive use of such compounds has led to the emergence of insecticide resistance in several Anopheles populations in Africa. This study aimed to investigate the presence of resistance alleles in an Anopheles arabiensis population from the City of Praia, capital of the Archipelago Cabo Verde, one of the countries on the World Health Organization list of countries that are on a path to eliminate local transmission of malaria.
METHODS:
Larvae from the Anopheles genus were collected using a one-pint dipper in three areas of City of Praia. Larvae were fed and maintained until the emergence of adult mosquitoes, and these were morphologically identified. In addition, molecular identification was performed using IGS markers and all An. arabiensis samples were subjected to PCR to screen for mutations associated to resistance in the Ace-1, Nav and GSTE2 genes.
RESULTS:
From a total of 440 mosquitoes collected, 52.3% were morphologically identified as An. gambiae sensu lato (s.l.) and 46.7% as Anopheles pretoriensis. The molecular identification showed that 100% of the An. gambiae s.l. were An. arabiensis. The mutations G119S in the Ace-1 gene and L119F in the GSTE2 gene were screened but not found in any sample. However, sequencing analysis for GSTE2 revealed the presence of 37 haplotypes, 16 polymorphic sites and a high genetic diversity (π = 2.67). The L1014S mutation in the Nav (voltage-gated sodium channel gene) was detected at a frequency of 7.3%.
CONCLUSION:
This is the first study to investigate the circulation of insecticide resistance alleles in An. arabiensis from Cabo Verde. The circulation of the L1014S allele in the population of An. arabiensis in the city of Praia suggests that pyrethroid resistance may arise, be quickly selected, and may affect the process of malaria elimination in Cabo Verde. Molecular monitoring of resistance should continue in order to guide the development of strategies to be used in vector control in the study region. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Ministério da Saúde. Instituto Nacional de Saúde Pública. Praia, Cabo Verde. | pt_BR |
Affilliation | Universidade Jean Piaget (UniPiaget). Praia, Cabo Verde. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Subject | Anopheles arabiensis | pt_BR |
Subject | Insecticide resistance | pt_BR |
Subject | L1014S kdr allele | pt_BR |
Subject | GSTE2 | pt_BR |
DeCS | Alelos | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Anopheles | pt_BR |
DeCS | Efeitos dos fármacos | pt_BR |
DeCS | Genética | pt_BR |
DeCS | Cabo Verde | pt_BR |
DeCS | Proteínas de Insetos | pt_BR |
DeCS | Metabolismo | pt_BR |
DeCS | Resistência a Inseticidas | pt_BR |
DeCS | Inseticidas | pt_BR |
DeCS | Farmacologia | pt_BR |
DeCS | Malária | pt_BR |
DeCS | Mosquitos Vetores | pt_BR |
DeCS | Reação em Cadeia da Polimerase | pt_BR |
DeCS | Piretrinas | pt_BR |
e-ISSN | 1475-2875 | pt_BR |