Author | Araújo, Ana Paula de | |
Author | Paiva, Marcelo Henrique Santos | |
Author | Cabral, Amanda Maria | |
Author | Cavalcanti, Antônio Emanuel Holanda Dias | |
Author | Pessoa, Luiz Fernando Freitas | |
Author | Diniz, Diego Felipe Araujo | |
Author | Helvecio, Elisama | |
Author | Silva, Ellyda Vanessa Gomes da | |
Author | Silva, Norma Machado da | |
Author | Anastácio, Daniela Bandeira | |
Author | Pontes, Claudenice | |
Author | Nunes, Vânia | |
Author | Souza, Maria de Fátima Marinho de | |
Author | Magalhães, Fernando Jorge Rodrigues | |
Author | Santos, Maria Alice Varjal de Melo | |
Author | Ayres, Constância Flávia Junqueira | |
Access date | 2019-09-24T14:23:47Z | |
Available date | 2019-09-24T14:23:47Z | |
Document date | 2019 | |
Citation | ARAÚJO, A. P. de et al. Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms. Journal of Insect Science, v. 19, n. 3, p. 1-15, 2019. | pt_BR |
ISSN | 1536-2442 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/35845 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms | pt_BR |
Type | Article | pt_BR |
DOI | 10.1093/jisesa/iez054 | |
Abstract | Resistance to chemical insecticides detected in Aedes aegypti (L.) mosquitoes has been a problem for the National Dengue Control Program (PNCD) over the last years. In order to provide deeper knowledge of resistance to xenobiotics, our study evaluated the susceptibility profile of temephos, diflubenzuron, and cypermethrin insecticides in natural mosquito populations from the Pernambuco State, associating these results with the local historical use of such compounds. Furthermore, mechanisms that may be associated with this particular type of resistance were characterized. Bioassays with multiple temephos and diflubenzuron concentrations were performed to detect and quantify resistance. For cypermethrin, diagnostic dose assays were performed. Biochemical tests were carried out to quantify the activity of detoxification enzymes. In addition, a screening of mutations present in the voltage-gated sodium channel gene (NaV) was performed in samples previously submitted to bioassays with cypermethrin. The populations under study were resistant to temephos and showed a positive correlation between insecticide consumption and the resistance ratio (RR) to the compound. For diflubenzuron, the biological activity ratio (BAR) ranged from 1.3 to 4.7 times, when compared to the susceptible strain. All populations showed resistance to cypermethrin. Altered enzymatic profiles of alpha, p-nitrophenyl acetate (PNPA) esterases and glutathione-S-transferases were recorded in most of these samples. Molecular analysis demonstrated that Arcoverde was the only population that presented the mutated form 1016Ile/Ile. These findings show that the situation is critical vis-à-vis the effectiveness of mosquito control using chemical insecticides, since resistance to temephos and cypermethrin is widespread in Ae. aegypti from Pernambuco. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Núcleo de Ciências da Vida. Caruaru, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética – BEG. Florianópolis, SC, Brasil. | pt_BR |
Affilliation | Departamento de Saúde de Pernambuco. Recife, PE, Brasil. | pt_BR |
Affilliation | Departamento de Saúde de Pernambuco. Recife, PE, Brasil. | pt_BR |
Affilliation | Secretaria Municipal de Saúde do Recife, Recife, PE, Brasil. | pt_BR |
Affilliation | Secretaria de Saúde do Distrito de Fernando de Noronha. Fernando de Noronha, PE, Brasil. | pt_BR |
Affilliation | Secretaria de Saúde do Distrito de Fernando de Noronha. Fernando de Noronha, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Subject | Insecticide resistance | pt_BR |
Subject | Chemical control | pt_BR |
Subject | Mosquito control | pt_BR |
DeCS | Aedes | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Diflubenzuron | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Resistência a Inseticidas | pt_BR |
DeCS | Genética | pt_BR |
DeCS | Inseticidas | pt_BR |
DeCS | Larva | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Piretrinas | pt_BR |
DeCS | Temefós | pt_BR |
DeCS | Testes de Toxicidade | pt_BR |
DeCS | Canais de Sódio Disparados por Voltagem | pt_BR |