Author | Gonçalves, Gabriel Gazzoni Araújo | |
Author | Feitosa, Ana Paula Sampaio | |
Author | Portela Júnior, Nairomberg Cavalcanti | |
Author | Oliveira, Cláudia Maria Fontes de | |
Author | Lima Filho, José Luiz de | |
Author | Brayner, Fábio André | |
Author | Alves, Luiz Carlos | |
Access date | 2019-09-19T14:35:08Z | |
Available date | 2019-09-19T14:35:08Z | |
Document date | 2019 | |
Citation | GONÇALVES, Gabriel Gazzoni Araújo et al. Use of MALDI-TOF MS to Identify the Culturable Midgut Microbiota of Laboratory and Wild Mosquitoes. Acta Tropica, p. 1-28, 13 Sept. 2019. | pt_BR |
ISSN | 1873-6254 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/35685 | |
Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brazil (CAPES) -Finance Code 001. PROEP-FIOCRUZ project (APQ-1656-2.13-15). Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco - Brasil (FACEPE). | pt_BR |
Language | eng | pt_BR |
Rights | restricted access | pt_BR |
Title | Use of MALDI-TOF MS to identify the culturable midgut microbiota of laboratory and wild mosquitoes | pt_BR |
Type | Preprint | pt_BR |
DOI | 10.1016/j.actatropica.2019.105174 | |
Abstract | Mosquitoes are responsible for transmitting many pathogens to humans and Aedes aegypti, Aedes albopictus and Culex quinquefasciatus are important vectors in the world. The microbiota plays an important role in developmental studies that involve impacts on the biological cycle of mosquitoes and vector control strategies. In this study, the aim was to understand the environment plays in the microbiota culturable diversity of Aedes aegytpi, Aedes albopictus and Culex quinquefasciatus. Midgut of studied mosquitoes (laboratory-reared and wild) were dissected and analyzed by MALDI-TOF MS to identify the microbiota. Most of the bacteria identified in the microbiota of mosquitoes from the laboratory and field belong to the phylum Proteobacteria. We reported on the microbial diversity among the mosquito species studied where Cx. quinquefasciatus and Ae. albopictus show greater bacterial similarity. The genus Rahnella was present in all mosquito species studied, both in those from the laboratory and those from the wild. Bacillus, Ewingella, Microccocus, Klebsiella and Pantoea are genera was predominant among the mosquitoes studied. The difference of microbiota diversity between mosquitoes laboratory-reared and wild shows that the environment plays an important role in the acquisition of bacteria, mainly in Ae. aegypti and Cx. quinquefasciatus. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keiso Asami (LIKA). Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keiso Asami (LIKA). Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keiso Asami (LIKA). Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keiso Asami (LIKA). Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keiso Asami (LIKA). Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keiso Asami (LIKA). Recife, PE, Brasil. | pt_BR |
Subject | Aedes aegypti | pt_BR |
Subject | Aedes albopictus | pt_BR |
Subject | Culex quinquefasciatus | pt_BR |
Subject | MALDI-TOF MS | pt_BR |
Subject | Microbiota | pt_BR |
Embargo date | 2050-01-01 | |