Author | Chaves, Anny Carolinny Tigre Almeida | |
Author | Rocha, Vinicius Pinto Costa | |
Author | Bastos, Tanira Matutino | |
Author | Soares, Milena Botelho Pereira | |
Author | Rodrigues, Ana Carolina Borges da Cruz | |
Author | Bezerra, Daniel Pereira | |
Author | Silva Júnior, Lauro José Caires | |
Author | Paula, Vanderlucia Fonseca de | |
Author | Silva, Edson Roberto da | |
Author | Queiroz, Raphael Ferreira | |
Access date | 2019-08-12T16:31:05Z | |
Available date | 2019-08-12T16:31:05Z | |
Document date | 2019 | |
Citation | CHAVES, Anny Carolinny Tigre Almeida et al. Antioxidant, antibacterial, leishmanicidal and trypanocidal activities of extract and fractions of Manilkara rufula stem bark. International Journal of Advanced Engineering Research and Science, v. 6, n. 6, p. 672-687, 2019. | pt_BR |
ISSN | 2349-6495 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34737 | |
Sponsorship | Fundação de Amparo a
Pesquisa do Estado da Bahia (FAPESB) and Conselho
Nacional de Desenvolvimento Científico Tecnológico
(CNPq). | pt_BR |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Sapotaceae | pt_BR |
Subject in Portuguese | Manilkara rufula | pt_BR |
Subject in Portuguese | Atividades farmacológicas | pt_BR |
Subject in Portuguese | Composição química | pt_BR |
Title | Antioxidant, antibacterial, leishmanicidal and trypanocidal activities of extract and fractions of Manilkara rufula stem bark | pt_BR |
Type | Article | pt_BR |
DOI | 10.22161/ijaers.6.6.78 | pt_BR |
Abstract | Manilkara rufula belongs to family Sapotaceae and has received little attention regarding its pharmacological properties and chemical composition. Therefore, our objective was to determine the farmacological activities and preliminary chemical profile of the stem bark from M. rufula. The plant stem bark of M. rufula was collected and ethanolic extract and fractions were prepared. The antioxidant activity was determined by •DPPH and ABTS•+ scavenger methods and inhibition of β-carotene oxidation. The minimum inhibitory and bactericidal concentrations were estimated through the broth microdilution method against gram positive and negative bacteria. Cytotoxicity, antioxidant and cytoprotection potential were investigated on
culture macrophages. Antiproliferative activity was evaluated by Alamar Blue method. The in vitro arginase activity from Leishmania amazonensis was determined in presence of the samples. In addition, their effects on survival of L. amazonensis and Trypanosoma cruzi amastigotes in mice macrophages were assessed. The preliminary phytochemical profile was evaluated by qualitative methods of classical phytochemistry, infrared
spectroscopy, LC-MS/MS and CG-MS. The samples presented antioxidant, bactericidal, leishmanicidal and trypanocidal properties without toxic characteristics on normal cells. Triterpenes were observed in the hexane
fraction, while glycosides, aromatic rings, fatty esters, proanthocyanidin dimers and trimers, catechin and various terpenes were observed in the other fractions. | pt_BR |
Affilliation | State University of the Southwest of Bahia. Department of Biological Sciences. Jequié, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | State University of the Southwest of Bahia. Department of Sciences and Technologies. Jequié, BA, Brazil. | pt_BR |
Affilliation | State University of the Southwest of Bahia. Department of Sciences and Technologies. Jequié, BA, Brazil. | pt_BR |
Affilliation | University of São Paulo. Faculty of Animal Science and Food Engineering. Pirassununga, SP, Brazil. | pt_BR |
Affilliation | State University of the Southwest of Bahia. Department of Natural Sciences. Vitória da Conquista, BA, Brazil. | pt_BR |
Subject | Sapotaceae | pt_BR |
Subject | Manilkara rufula | pt_BR |
Subject | Pharmacological activities | pt_BR |
Subject | Chemical composition | pt_BR |