Author | Contessoto, Nayara Sousa de Alcântara | |
Author | Caruso, Ícaro Putinhon | |
Author | Bezerra, Daniel Pereira | |
Author | Barbosa Filho, José Maria | |
Author | Cornélio, Marinônio Lopes | |
Access date | 2019-08-09T17:17:43Z | |
Available date | 2019-08-09T17:17:43Z | |
Document date | 2019 | |
Citation | CONTESSOTO, Nayara Sousa de Alcântara et al. An investigation into the interaction between piplartine (piperlongumine) and human serum albumin. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 220, p. 1-7, 2019. | pt_BR |
ISSN | 1386-1425 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34697 | |
Sponsorship | Conselho Nacional de Desenvol. Científico e Tecnológico (CNPq), Brazil - (Grant 130449/2015-6 and 141714/2017-4). MLC and NSAC acknowledge the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Brazil - (Grant 2017/08834-9). | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Piperidina | pt_BR |
Subject in Portuguese | Piperlongumine | pt_BR |
Subject in Portuguese | Albumina humana sérica | pt_BR |
Subject in Portuguese | Espectroscopia de Fluorescência | pt_BR |
Subject in Portuguese | Ancoragem Molecular | pt_BR |
Title | An investigation into the interaction between piplartine (piperlongumine) and human serum albumin | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.saa.2019.04.076 | |
Abstract | Piplartines are alkaloid amides present in the roots and stems of different pepper species which have promising pharmacological properties including cancer prevention. Some recent studies have determined pharmacokinetic parameters of piplartine in rat blood plasma but without pointing to any molecular target or describing the physicochemical forces of the interaction. The present study investigated the interaction between piplartine and human serum albumin (HSA) the predominant protein in blood plasma. Fluorescence spectroscopy was utilized to observe the complex HSA-piplartine formation. Thermodynamic parameter analysis indicates that the process occurs spontaneously and is enthalpically driven; the affinity constant suggests that this interaction is reversible. This was reinforced by the binding density function method and by the displacement analysis that the piplartine binds on HSA at a single site, which was determined to be the IIA sub-domain. In silico analysis (molecular docking) identified the main residues involved in binding and the corresponding forces, which corroborates well with the experimental results. | pt_BR |
Affilliation | Universidade Estadual de São Paulo. Instituto de Biociências. Departamento de Física. Letras e Ciências Exatas. São José do Rio Preto, SP, Brasil. | pt_BR |
Affilliation | Universidade Estadual de São Paulo. Instituto de Biociências. Departamento de Física. Letras e Ciências Exatas. São José do Rio Preto, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil. | pt_BR |
Affilliation | Universidade Estadual de São Paulo. Instituto de Biociências. Departamento de Física. Letras e Ciências Exatas. São José do Rio Preto, SP, Brasil. | pt_BR |
Subject | Piplartine | pt_BR |
Subject | Piperlongumine | pt_BR |
Subject | Human serum albumin | pt_BR |
Subject | Fluorescence spectroscopy | pt_BR |
Subject | Molecular docking | pt_BR |