Author | Mata, Verónica Elizabeth | |
Author | Passos, Sonia Regina Lambert | |
Author | Hökerberg, Yara Hahr Marques | |
Author | Berardinelli, Guilherme Miguéis | |
Author | Santos, Maria Angelica Borges dos | |
Author | Fukuoka, Levy Vilas Boas | |
Author | Maciel, Anna Carolina Fontoura Seixas Rangel | |
Author | Rodrigues, Cintia Damasceno dos Santos | |
Author | Santos, Aline da Silva | |
Author | Oliveira, Raquel de Vasconcellos Carvalhaes de | |
Access date | 2019-08-07T13:25:15Z | |
Available date | 2019-08-07T13:25:15Z | |
Document date | 2017 | |
Citation | MATA, Verónica Elizabeth et al. Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory. BMC Infectious Diseases, v. 17, p. 1-7, 2017. | pt_BR |
ISSN | 1471-2334 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34647 | |
Description | Inclui correção. | pt_BR |
Language | eng | pt_BR |
Publisher | BMC | pt_BR |
Rights | open access | pt_BR |
Title | Accuracy and reliability of an NS1 rapid immunochromatographic test for DENV-1 diagnosis at point of care and in the laboratory | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s12879-017-2679-z | |
Abstract | Background: Rapid immunochromatographic tests (ICT) for dengue non-structural protein 1 (NS1) have shown good performance for diagnosing acute-phase dengue in serum in laboratory settings, but rarely have been assessed in whole blood and at point of care (POC). This study compare the accuracy and inter- and intra-observer reliability of the NS1 Bioeasy™ ICT in whole blood at POC versus serum in the laboratory, during a DENV-1 epidemic. Methods: Cross-sectional study involving 144 adults spontaneously demanding care in an emergency department within 4 days of onset of acute febrile illness. Accuracy of NS1 Bioeasy™ ICT was compared in whole blood and serum, both at 15 and 30 min, blinded to the reference RT-PCR or NS1 ELISA. Non-dengue patients were also tested for Zika virus with RT-PCR. Reliability of whole blood and serum readings by the same or different observers was measured by simple kappa (95% CI).
Results: At 15 min, sensitivity (Sn) of NS1 Bioeasy™ ICT in whole blood/POC was 76.7% (95% CI: 68.0–84.1) and specificity (Sp) was 87.0% (95% CI: 66.4–97.2). Sn in serum/laboratory was 82% (95% CI: 74.1–88.6) and Sp 100% (95% CI: 85.8–100). Positive likelihood ratio was 5.9 (95% CI: 2.0–17.0) for whole blood/POC and 19.8 (95% CI: 2.9–135.1) for serum/laboratory. Reliability of matched readings of whole blood/POC and serum/laboratory by the same observer (k = 0.83, 95% CI: 0.74–0.93) or different observers (k = 0.81, 95% CI: 0.72–0.92) was almost perfect, with higher discordant levels in the absence of dengue. Results did not differ statistically at 5%. Conclusions: NS1 Bioeasy™ ICT in DENV-1 epidemics is a potentially confirmatory test. Invalid results at 15 min should be reread at 30 min. To optimize impact of implementing ICT in the management of false-negatives it should be incorporated into an algorithm according to setting and available specimen. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Sem afiliação. | pt_BR |
Affilliation | Sem afiliação. | pt_BR |
Affilliation | Sem afiliação. | pt_BR |
Affilliation | Sem afiliação. | pt_BR |
Affilliation | Sem afiliação. | pt_BR |
Affilliation | Sem afiliação. | pt_BR |
Affilliation | Sem afiliação. | pt_BR |
Affilliation | Sem afiliação. | pt_BR |
Affilliation | Sem afiliação. | pt_BR |
Subject | Dengue | pt_BR |
Subject | Sensitivity and specificity | pt_BR |
Subject | Diagnosis | pt_BR |
Subject | Point-of-care systems | pt_BR |
Subject | NS1 | pt_BR |
Subject | Reproducibility of results | pt_BR |
e-ISSN | 1471-2334 | |