Author | Allahdadi, Kyan James | |
Author | Santana, Thaís Alves de | |
Author | Santos, Girlaine Café | |
Author | Azevedo, Carine Machado | |
Author | Mota, Roberta Alves | |
Author | Nonaka, Carolina Kymie | |
Author | Silva, Daniela Nascimento | |
Author | Valim, Clarissa Xavier Resende | |
Author | Figueira, Cláudio Pereira | |
Author | dosSantos, Washington Luis Conrado | |
Author | Espirito Santo, Renan Fernandes do | |
Author | Evangelista, Afrânio Ferreira | |
Author | Villarreal, Cristiane Flora | |
Author | Santos, Ricardo Ribeiro dos | |
Author | Souza, Bruno Solano Freitas de | |
Author | Soares, Milena Botelho Pereira | |
Access date | 2019-08-06T17:35:12Z | |
Available date | 2019-08-06T17:35:12Z | |
Document date | 2019 | |
Citation | ALLAHDADI, Kyan James et al. IGF-1 overexpression improves mesenchymal stem cell survival and promotes neurological recovery after spinal cord injury. Stem Cell Research & Therapy, v. 10, p. 1-14, 2019. | pt_BR |
ISSN | 1757-6512 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34630 | |
Sponsorship | Brazilian agencies: The
National Bank for Economic and Social Development (BNDES), the National
Research Council (CNPq), the Foundation of Support for Research of the
State of Bahia (FAPESB) and Funding Authority for Studies and Projects
(FINEP). | pt_BR |
Language | eng | pt_BR |
Publisher | BMC | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Lesão medular | pt_BR |
Subject in Portuguese | Células-tronco mesenquimais derivadas da medula óssea | pt_BR |
Subject in Portuguese | IGF-1 | pt_BR |
Subject in Portuguese | Terapia gênica e celular | pt_BR |
Title | IGF-1 overexpression improves mesenchymal stem cell survival and promotes neurological recovery after spinal cord injury | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s13287-019-1223-z | |
Abstract | Survival and therapeutic actions of bone marrow-derived mesenchymal stem cells (BMMSCs) can be limited by the hostile microenvironment present during acute spinal cord injury (SCI). Here, we investigated whether BMMSCs overexpressing insulin-like growth factor 1 (IGF-1), a cytokine involved in neural development and injury repair, improved the therapeutic effects of BMMSCs in SCI. Methods: Using a SCI contusion model in C57Bl/6 mice, we transplanted IGF-1 overexpressing or wild-type
BMMSCs into the lesion site following SCI and evaluated cell survival, proliferation, immunomodulation, oxidative stress, myelination, and functional outcomes. Results: BMMSC-IGF1 transplantation was associated with increased cell survival and recruitment of endogenous neural progenitor cells compared to BMMSC- or saline-treated controls. Modulation of gene expression of pro- and anti-inflammatory mediators was observed after BMMSC-IGF1 and compared to saline- and BMMSC-treated mice. Treatment with BMMSC-IGF1 restored spinal cord redox homeostasis by upregulating antioxidant defense genes. BMMSC-IGF1 protected against SCI-induced myelin loss, showing more compact myelin 28 days after SCI.
Functional analyses demonstrated significant gains in BMS score and gait analysis in BMMSC-IGF1, compared to BMMSC or saline treatment. Conclusions: Overexpression of IGF-1 in BMMSC resulted in increased cell survival, immunomodulation, myelination, and functional improvements, suggesting that IGF-1 facilitates the regenerative actions of BMMSC in
acute SCI. | pt_BR |
Affilliation | Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil / São Rafael Hospital. D’Or Institute for Research and Education. Salvador, BA, Brazil. | pt_BR |
Affilliation | Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
Affilliation | Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
Affilliation | Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
Affilliation | Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. D’Or Institute for Research and Education. Salvador, BA, Brazil. | pt_BR |
Affilliation | Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brazil. | pt_BR |
Affilliation | Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil / São Rafael Hospital. D’Or Institute for Research and Education. Salvador, BA, Brazil. | pt_BR |
Affilliation | Hospital São Rafael. Center for Biotechnology and Cell Therapy. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil. | pt_BR |
Subject | Spinal cord injury | pt_BR |
Subject | Bone marrow-derived mesenchymal stem cells | pt_BR |
Subject | IGF-1 | pt_BR |
Subject | Gene and cell therapy | pt_BR |