Author | Guimarães, Erick Vaz | |
Author | Carvalho, Laís de | |
Author | Barbosa, Helene Santos | |
Access date | 2019-07-23T12:56:09Z | |
Available date | 2019-07-23T12:56:09Z | |
Document date | 2003 | |
Citation | GUIMARÃES, Erick Vaz; CARVALHO, Laís de; BARBOSA, Helene Santos. An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 98, n. 7, p. 915-917, Oct. 2003. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/34338 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Toxoplasma gondii | pt_BR |
Subject in Portuguese | Citoquímica ultraestrutural | pt_BR |
Subject in Portuguese | Grânulos de amilopectina | pt_BR |
Subject in Portuguese | Cistos teciduais | pt_BR |
Title | An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/s0074-02762003000700010 | |
Abstract | Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Histologia e Embriologia. Laboratório de Cultura de Células. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | Toxoplasma gondii | pt_BR |
Subject | Amylopectin granules | pt_BR |
Subject | Tissue cysts | pt_BR |
Subject | Ultrastructural cytochemistry | pt_BR |
e-ISSN | 1678-8060 | |