Author | Tendler, Miriam | |
Author | Pinto, Roberto Magalhães | |
Author | Lima, Antonio de Oliveira | |
Author | Savino, Wilson | |
Author | Katz, Naftale | |
Access date | 2019-06-23T15:09:06Z | |
Available date | 2019-06-23T15:09:06Z | |
Document date | 1992 | |
Citation | TENDLER, Miriam et al. Vaccination in murine schistosomiasis with adult worm derived antigens - II. Protective and immune response in inbred mice. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, Suppl. 1, p. 281-286, 1992. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/33623 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Vacinação | pt_BR |
Subject in Portuguese | Schistosoma mansoni | pt_BR |
Subject in Portuguese | Antígenos | pt_BR |
Subject in Portuguese | Modelo murino | pt_BR |
Title | Vaccination in murine schistosomiasis with adult worm derived antigens - II. Protective and immune response in inbred mice | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02761992000500053 | pt_BR |
Abstract | Previous studies have shown that both permissive (mouse) and partially permissive (rabbit) hosts develop high levels of resistance against Schistosoma mansoni infection after vaccination with a multiple antigen extract (SE) obtained by incubation of living adult worms in saline, plus bacterial adjuvant. To investigate variables influencing SE-induced protection in murine schistosomiasis, a series of distinct vaccination protocols were performed focussing on the immunization dose, carrier systems, route, site and amplitude of challenge infection, and time between immunization and challenge. In addition, a new approach was adopted to evaluate SE protective activity, by means of population analysis of worm burden frequency distributions in a large scale study of vaccination in outbred Swiss mice. Distinct curves of frequency and a drastic difference in worm burden distribution of frequencies from SE-vaccinated × non-vaccinated mice were found. It was shown that SE could generate 75% mean protection in outbred mice even in the absence of adjuvant. In addition SE immunization was also able to induce full protection against lethal infection. SE-induced protection could be modulated by such parameters as dose of SE immunization/challenge interval, and route of cercariae injection. These data show that SE yields very high protective activity in outbred mice, and may provide a further insight for rational design of a vaccine in experimental schistosomiasis. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Ataulpho de Paiva. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil | pt_BR |
Subject | Vaccination | pt_BR |
Subject | Schistosoma mansoni | pt_BR |
Subject | Murine model | pt_BR |
Subject | Antigens | pt_BR |
e-ISSN | 1678-8060 | |