Author | Pereira, Isabela Tiemy | |
Author | Spangenberg, Lucia | |
Author | Robert, Anny Waloski | |
Author | Amorín, Rocío | |
Author | Stimamiglio, Marco Augusto | |
Author | Naya, Hugo | |
Author | Dallagiovanna, Bruno | |
Access date | 2019-05-17T19:42:36Z | |
Available date | 2019-05-17T19:42:36Z | |
Document date | 2019 | |
Citation | PEREIRA, Isabela Tiemy et al. Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes. BMC Genomics, v. 20, n. 219, p. 1-16, 2019. | pt_BR |
ISSN | 1471-2164 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/33134 | |
Language | eng | pt_BR |
Publisher | BMC | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Cardiomogênese | pt_BR |
Subject in Portuguese | Perfil Polysome | pt_BR |
Title | Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s12864-019-5550-3 | |
Abstract | Cardiac cell fate specification occurs through progressive steps, and its gene expression regulation features are still being defined. There has been an increasing interest in understanding the coordination between transcription and post-transcriptional regulation during the differentiation processes. Here, we took advantage of the polysome profiling technique to isolate and high-throughput sequence ribosome-free and polysome-bound RNAs during cardiomyogenesis. We showed that polysome-bound RNAs exhibit the cardiomyogenic commitment gene expression and that mesoderm-to-cardiac progenitor stages are strongly regulated. Additionally, we compared ribosome-free and polysome-bound RNAs and found that the post-transcriptional regulation vastly contributes to cardiac phenotype determination, including RNA recruitment to and dissociation from ribosomes. Moreover, we found that protein synthesis is decreased in cardiomyocytes compared to human embryonic stem-cells (hESCs), possibly due to the down-regulation of translation-related genes. Our data provided a powerful tool to investigate genes potentially controlled by post-transcriptional mechanisms during the cardiac differentiation of hESC. This work could prospect fundamental tools to develop new therapy and research approaches. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Subject | Cardiomyogenesis | pt_BR |
Subject | Cardiomyocytes | pt_BR |
Subject | Polyribosomes | pt_BR |
Subject | Gene Expression Regulation | pt_BR |
Subject | RNA Processing, Post-Transcriptional | pt_BR |
Subject in Spanish | Miocitos Cardíacos | pt_BR |
Subject in Spanish | Polirribosomas | pt_BR |
Subject in Spanish | Regulación de la Expresión Génica | pt_BR |
Subject in Spanish | Procesamiento Postranscripcional del ARN | pt_BR |
DeCS | Miócitos Cardíacos | pt_BR |
DeCS | Polirribossomos | pt_BR |
DeCS | Regulação da Expressão Gênica | pt_BR |
DeCS | Processamento Pós-Transcricional do RNA | pt_BR |
e-ISSN | 1471-2164 | |