Author | Silva, Siuhelem Rocha | |
Author | Almeida, Anne Cristine Gomes | |
Author | Silva, George Allan Villarouco da | |
Author | Ramasawmy, Rajendranath | |
Author | Lopes, Stefanie Costa Pinto | |
Author | Siqueira, André Machado | |
Author | Costa, Gabriel Luíz | |
Author | Sousa, Taís Nóbrega | |
Author | Vieira, José Luiz Fernandes | |
Author | Lacerda, Marcus Vinícius Guimarães | |
Author | Monteiro, Wuelton Marcelo | |
Author | Melo, Gisely Cardoso de | |
Access date | 2019-05-03T12:32:18Z | |
Available date | 2019-05-03T12:32:18Z | |
Document date | 2018 | |
Citation | SILVA, Siuhelem Rocha et al. Chloroquine resistance is associated to multi-copy pvcrt-o gene in Plasmodium vivax malaria in the Brazilian Amazon. Malaria Journal, v. 17, n. 1, p. 1-8, 2018. | pt_BR |
ISSN | 1475-2875 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32927 | |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Plasmodium vivax | pt_BR |
Subject in Portuguese | Malaria | pt_BR |
Title | Chloroquine resistance is associated to multi-copy pvcrt-o gene in Plasmodium vivax malaria in the Brazilian Amazon | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s12936-018-2411-5 | |
Abstract | BACKGROUND: The resistance of Plasmodium vivax to chloroquine has become an obstacle to control strategies based on the use of anti-malarials. The current study investigated the association between P. vivax CQ-resistance in vivo with copy number variation and mutations in the promoter region in pvcrt-o and pvmdr1 genes. METHODS: The study included patients with P. vivax that received supervised treatment with chloroquine and primaquine. Recurrences were actively recorded during this period.
RESULTS: Among the 60 patients with P. vivax, 25 were CQ-resistant and 35 CQ-susceptible. A frequency of 7.1% of multi-copy pvcrt-o was observed in CQ-susceptible samples and 7.7% in CQ-resistant at D0 (P > 0.05) and 33.3% in CQ-resistant at DR (P < 0.05). For pvmdr1, 10.7% of the CQ-susceptible samples presented multiple copies compared to 11.1% in CQ-resistant at D0 and 0.0% in CQ-resistant at DR (P > 0.05). A deletion of 19 bp was found in 11/23 (47.6%) of the patients with CQ-susceptible P. vivax and 3/10 (23.1%) of the samples with in CQRPv at D0. At day DR, 55.5% of the samples with CQRPv had the 19 bp deletion. For the pvmdr-1 gene, was no variation in the analysed gene compared to the P. vivax reference Sal-1.
CONCLUSIONS: This was the first study with 42-day clinical follow-up to evaluate the variation of the number of copies and polymorphisms in the promoter region of the pvcrt-o and pvmdr1 genes in relation to treatment outcomes. Significantly higher frequency of multi-copy pvcrt-o was found in CQRPv samples at DR compared to CQ-susceptible, indicating parasite selection of this genotype after CQ treatment and its association with CQ-resistance in vivo. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade do Estado do Amazonas. Manaus, AM, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade do Estado do Amazonas. Manaus, AM, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade do Estado do Amazonas. Manaus, AM, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade do Estado do Amazonas. Manaus, AM, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal do Pará. Belém, PA, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade do Estado do Amazonas. Manaus, AM, Brazil. | pt_BR |
Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil / Universidade do Estado do Amazonas. Manaus, AM, Brazil. | pt_BR |
Subject | Plasmodium vivax | pt_BR |
Subject | Malaria | pt_BR |
Embargo date | 2030-12-31 | |