Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/32899
Type
ArticleCopyright
Restricted access
Embargo date
2022-01-01
Collections
- IOC - Artigos de Periódicos [12500]
Metadata
Show full item record
HIGH PREVALENCE OF ENTERIC VIRUSES ASSOCIATED WITH ACUTE GASTROENTERITIS IN PEDIATRIC PATIENTS IN A LOW-INCOME AREA IN VITÓRIA, SOUTHEASTERN BRAZIL
Gastroenterite aguda
Vírus entéricos
Norovirus
Análise filogenética
Adenovírus
Adenovirus
Enteric viruses
Norovirus
Pphylogenetic analysis
Rotavirus
Author
Affilliation
Universidade Federal do Espírito Santo. Laboratório de Saneamento. Vitória, ES, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Espírito Santo. Laboratório de Epidemiologia. Vitória, ES, Brasil.
Universidade Federal do Espírito Santo. Laboratório de Saneamento. Vitória, ES, Brasil.
Universidade Federal do Espírito Santo. Laboratório de Saneamento. Vitória, ES, Brasil.
Universidade Federal do Espírito Santo. Laboratório de Saneamento. Vitória, ES, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Espírito Santo. Laboratório de Epidemiologia. Vitória, ES, Brasil.
Universidade Federal do Espírito Santo. Laboratório de Saneamento. Vitória, ES, Brasil.
Universidade Federal do Espírito Santo. Laboratório de Saneamento. Vitória, ES, Brasil.
Universidade Federal do Espírito Santo. Laboratório de Saneamento. Vitória, ES, Brasil.
Abstract
Acute gastroenteritis (AGE) is a significant cause of child mortality worldwide. In Brazil, despite the reduction in infant mortality achieved in recent years, many children still die because of undiagnosed AGE. The prevalence, viral load, and circulating genotypes of rotavirus A (RVA), human adenovirus (HAdV), and norovirus GII (NoV GII) were investigated in children with AGE during 12 months in Vitoria, Espírito Santo, Southeastern Brazil. Enteric viruses were detected in stool samples, quantified by quantitative polymerase chain reaction, sequenced, and compared phylogenetically. The overall prevalence was 93.3% (125/134). Cases of single infection (41.8%) and mixed infection (51.5%) were observed; in 21.6% of cases, all the three viruses were detected. RVA had the highest number of copies in all infections. Phylogenetic analysis revealed predominantly the presence of RVA genotype G3, followed by G2 and G9. HAdV clustered within subgroup C, but some samples harbored subgroups A, D, or F. All sequenced NoV-positive samples clustered within the prevalent genotype GII.4. The high prevalence of RVA, HAdV, and NoV in diarrheal feces clarifies the etiology of AGE in this population, and the presence of RVA in vaccinated children reinforces the importance of monitoring programs to identify the causes of gastroenteritis and contribute to the reliability of diagnosis.
Keywords in Portuguese
RotavírusGastroenterite aguda
Vírus entéricos
Norovirus
Análise filogenética
Adenovírus
Keywords
Acute gastroenteritisAdenovirus
Enteric viruses
Norovirus
Pphylogenetic analysis
Rotavirus
Share