Author | Martins, Hariany Seabra | |
Author | Silva, Grazielle Carolina da | |
Author | Cortes, Steiner Franca | |
Author | Leme, Fabiola de Oliveira Paes | |
Author | Martins Filho, Olindo Assis | |
Author | Araújo, Márcio Sobreira Silva | |
Author | Stahlberg, Rubens | |
Author | Lagares, Monique de Albuquerque | |
Access date | 2019-04-26T15:16:24Z | |
Available date | 2019-04-26T15:16:24Z | |
Document date | 2018 | |
Citation | MARTINS, Hariany Seabra et al. Lactoferrin increases sperm membrane functionality of frozen equine semen. Reproduction in domestic animals, v. 53, p. 617–623, 2018. | pt_BR |
ISSN | 0936-6768 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32784 | |
Language | eng | pt_BR |
Publisher | Blackwell Publishing | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Antioxidante | pt_BR |
Subject in Portuguese | Catalase | pt_BR |
Subject in Portuguese | Criopreservação | pt_BR |
Subject in Portuguese | Cavalo | pt_BR |
Subject in Portuguese | Espermatozoides | pt_BR |
Subject in Portuguese | Garanhão | pt_BR |
Title | Lactoferrin increases sperm membrane functionality of frozen equine semen | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/rda.13148 | |
Abstract | Contents During cryopreservation, sperm was submitted to an increase in reactive oxygen species generation. This work aimed to improve the quality of frozen equine sperm after the addition of antioxidants lactoferrin (Lf) and catalase (Cat) to a freezing extender. Semen from six stallions was frozen with the extenders: F1) control, INRA 82 freezing extender, F2) F1+500g/ml Lf and F3) F1+200 IU/ml Cat. After thawing, sperm motility parameters, membrane functionality and integrity, and acrosome integrity and spontaneous acrosome-reacted sperm were evaluated with a computer-assisted sperm analysis, a hypoosmotic swelling test and epifluorescent microscopy, respectively. Nitrite, hydroperoxide and iron concentrations of frozen semen were measured with spectrophotometry. The percentage of functional membrane sperm treated with Lf was higher (50.7%+/- 11.6%) compared to that of the control (37.6%+/- 15.6%), while the iron (61.4 +/- 11.6 vs 73.3 +/- 13.8 mg/dl) and nitrite concentrations (16.3 +/- 7.1 vs 25.9 +/- 4.2M/g protein) were lower, respectively (p<.05). Thus, it can be suggested that Lf protect stallion spermatozoon during freezing as it has increased the percentage of sperm with functional membrane and decreased the lipid oxidant agents. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clinica e Cirurgia Veterinárias. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Farmacologia. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Farmacologia. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clinica e Cirurgia Veterinárias. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Biomarcadores de Diagnostico e Monitoração. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Biomarcadores de Diagnostico e Monitoração. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Pontifícia Universidade Católica de Minas Gerais. Faculdade de Medicina Veterinária. Betim, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clinica e Cirurgia Veterinárias. Belo Horizonte, MG, Brazil. | pt_BR |
Subject | Antioxidant | pt_BR |
Subject | Catalase | pt_BR |
Subject | Cryopreservation | pt_BR |
Subject | Horse | pt_BR |
Subject | Spermatozoa | pt_BR |
Subject | Stallion | pt_BR |
Embargo date | 2150-01-01 | |