Author | Santos, Fabiana Cristina Fulco | |
Author | Lira, Laís Ariane de Siqueira | |
Author | Montenegro, Rosana de Albuquerque | |
Author | Lima, Juliana Figueirêdo da Costa | |
Author | Lima, Andrea Santos | |
Author | Schindler, Haiana Charifker | |
Author | Montenegro, Lílian Maria Lapa | |
Access date | 2019-04-22T13:30:48Z | |
Available date | 2019-04-22T13:30:48Z | |
Document date | 2018 | |
Citation | SANTOS, F. C. F. et al. Performance of the IS6110-TaqMan® Assay in the Diagnosis of Extrapulmonary Tuberculosis from Different Biological Samples. Revista da Sociedade Brasileira de Medicina Tropical, v. 51, n. 3, p. 331-337, jun. 2018. | pt_BR |
ISSN | 1678-9849 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32656 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/0037-8682-0372-2017 | |
Abstract | Introduction: This study evaluated the performance of the IS6110-TaqMan® assay in different types of biological samples and
tissues for laboratory diagnosis of extrapulmonary tuberculosis. Methods: 143 biological samples and tissues from patients
with suspected extrapulmonary tuberculosis from the health services of Recife/Pernambuco/Brazil were evaluated with the
IS6110-TaqMan® assay. Results: The sensitivities of the IS6110-TaqMan® assay calculated for blood, urine, both blood and
urine samples, tissue biopsies, extrapulmonary body fluid samples, and all samples from patients calculated together were 55.9%,
33.3%, 68.8%, 43.8%, 29.6%, and 73.7%, respectively, and the specificities were 80%, 100%, 78.6%, 100%, 100%, and 84.2%,
respectively. Conclusions: The accuracy of qPCR was high in various clinical sample types. The analysis of more than one
type of clinical sample collected from the same patient with extrapulmonary tuberculosis enhances the diagnostic power of the
IS6110-TaqMan® assay when compared with the use of only one clinical sample. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. | pt_BR |
Subject | Mycobacterium tuberculosis complex | pt_BR |
Subject | IS6110-TaqMan® assay | pt_BR |
Subject | Molecular diagnosis | pt_BR |
Subject | Extrapulmonary tuberculosis | pt_BR |
DeCS | DNA bacteriano / análise | pt_BR |
DeCS | DNA Bacteriano / genética | pt_BR |
DeCS | DNA bacteriano / isolamento e purificação | pt_BR |
DeCS | Método Duplo-cego | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Mycobacterium tuberculosis / genética | pt_BR |
DeCS | Mycobacterium tuberculosis / isolamento & purificação | pt_BR |
DeCS | Estudos prospectivos | pt_BR |
DeCS | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
DeCS | Reprodutibilidade dos resultados | pt_BR |
DeCS | Sensibilidade e Especificidade | pt_BR |
DeCS | Tuberculose / diagnóstico | pt_BR |