Author | Oliveira, Camila Indiani de | |
Author | Teixeira, Maria Jânia | |
Author | Teixeira, Clarissa Romero | |
Author | Jesus, Joílson Ramos de | |
Author | Rosato, Andréa Bomura | |
Author | Silva, João Santana da | |
Author | Brodskyn, Claudia Ida | |
Author | Barral Netto, Manoel | |
Author | Barral, Aldina Maria Prado | |
Access date | 2011-10-18T20:41:18Z | |
Available date | 2011-10-18T20:41:18Z | |
Document date | 2004 | |
Citation | OLIVEIRA, Camila Indiani de et al. Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice. Microbes Infection, v. 6, n. 11, p. 977-984, Sept. 2004. | pt_BR |
ISSN | 1286-4579 | |
URI | https://www.arca.fiocruz.br/handle/icict/3264 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.micinf.2004.05.009 | |
Abstract | Leishmania braziliensis is the species responsible for the majority of cases of human cutaneous leishmaniasis in Brazil. In the present study,
L. braziliensis isolates from two different geographic areas in Brazil were studied by RAPD, using arbitrary primers.We also evaluated other
biological features of these two isolates. We compared (a) the clinical features they initiate or not once delivered subcutaneously as
stationary-phase promastigotes in the footpad of BALB/c mice; (b) the parasite load in both the footpad and the draining lymph node; (c) the
cytokines present in the supernatant of cultures of the cell suspensions from the draining lymph nodes; and (d) the cell types present at the site
of parasite delivery. The results show that the L. braziliensis strain from Ceará (H3227) is genotypically different from the L. braziliensis strain
from Bahia (BA788). H3227-parasitized mice developed detectable lesions, whereas BA788-parasitized mice did not. Fifteen days post
parasite inoculation there was an increase in the numbers of macrophages and lymphocytes in the footpads, whatever the parasite inoculum.
Parasite load at the inoculation site—namely the footpad—did not differ significantly; in draining lymph nodes, however, it increased over the
period under study. Early after parasite inoculation, the cells recovered from the draining lymph nodes of BA788-parasitized mice produced
higher levels of IFN-c, a feature coupled to a higher number of NK cells. Later, after the parasite inoculation, there was an increased content
of IL-12p70 and IL-10 in the supernatant of cells recovered from the lymph nodes of H3227-parasitized mice. This comparative analysis points
out that L. braziliensis isolates differing in | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil. | pt_BR |
Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto, São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brazil. | pt_BR |
Subject | Leishmania braziliensis | pt_BR |
Subject | Random amplification of polymorphic DNA | pt_BR |
Subject | Interferon gamma | pt_BR |
Subject | Interleukin-12p70 | pt_BR |
Subject | Interleukin-10 | pt_BR |
DeCS | Células Cultivadas | pt_BR |
DeCS | Citocinas | pt_BR |
DeCS | Impressões Digitais de DNA | pt_BR |
DeCS | DNA de Protozoário | pt_BR |
DeCS | Modelos Animais de Doenças | pt_BR |
DeCS | Genoma de Protozoário | pt_BR |
DeCS | Interferon gama | pt_BR |
DeCS | Interleucina 10 | pt_BR |
DeCS | Interleucina-12 | pt_BR |
DeCS | Celulas Matadoras Naturais | pt_BR |
DeCS | Leishmania braziliensis | pt_BR |
DeCS | Leishmaniose Cutânea | pt_BR |
DeCS | Linfonodos | pt_BR |
DeCS | Camundongos Endogâmicos BALB C | pt_BR |
DeCS | Polimorfismo Genético | pt_BR |
DeCS | Subunidades Proteícas | pt_BR |
DeCS | Técnica de Amplificação ao Acaso de DNA Polimórfico | pt_BR |