Author | Carvalhal, Djalma Gomes Ferrão | |
Author | Barbosa Junior, Aryon de Almeida | |
Author | Hermida, Micely D' El Rei | |
Author | Clarêncio, Jorge | |
Author | Pinheiro Junior, Nathanael F | |
Author | Veras, Patrícia Sampaio Tavares | |
Author | dosSantos, Washington Luis Conrado | |
Access date | 2011-10-18T18:57:47Z | |
Available date | 2011-10-18T18:57:47Z | |
Document date | 2004 | |
Citation | CARVALHAL, D.G. et al. The modelling of mononuclear phagocyte-connective tissue adhesion in vitro: application to disclose a specific inhibitory effect of Leishmania infection. Experimental Parasitology, v. 107, n. 3-4, p. 189-199, jul.-aug. 2004. | pt_BR |
ISSN | 0014-4894 | |
URI | https://www.arca.fiocruz.br/handle/icict/3263 | |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | The modelling of mononuclear phagocyte-connective tissue adhesion in vitro: application to disclose a specific inhibitory effect of Leishmania infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.exppara.2004.06.003 | |
Abstract | In this work, we have developed an adhesion assay to study interactions between mononuclear phagocytes and connective tissue in vitro and show its potential use to study diseases caused by intracellular microorganisms. The assay reproduces most of the characteristics of macrophage adhesion to connective tissue in vivo, such as: preferential adhesion to inflamed connective tissue, divalent cation and integrin dependence, and up-regulation upon cell activation. The phagocyte adhesion to connective tissue was inhibited by infection with Leishmania (58+/-22%, p < 0.05) and was not affected by infection with Mycobacterium or by endocytosis of latex beads. Manganese partially reverted the loss in adherence produced by Leishmania infection, indicating that the mechanisms regulating the function of integrins are affected by cell infection with Leishmania. This assay might be a useful tool for the study of the mechanisms by which mononuclear phagocytes play a role in the immune-inflammatory response and in the development of lesions. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Subject | Adhesion assay | pt_BR |
Subject | Cell adhesion | pt_BR |
Subject | Macrophage adhesion | pt_BR |
Subject | J774 cells | pt_BR |
Subject | Leishmania amazonensis | pt_BR |
Subject | Leishmania braziliensis | pt_BR |
Subject | Promastigotes | pt_BR |
DeCS | Análise de Variância | pt_BR |
DeCS | Antígenos CD | pt_BR |
DeCS | Cátions Bivalentes | pt_BR |
DeCS | Adesão Celular | pt_BR |
DeCS | Linhagem Celular | pt_BR |
DeCS | Tecido Conjuntivo | pt_BR |
DeCS | Técnicas de Cultura | pt_BR |
DeCS | Inflamação | pt_BR |
DeCS | Integrinas | pt_BR |
DeCS | Leishmanias | pt_BR |
DeCS | Leucócitos Mononucleares | pt_BR |
DeCS | Manganês | pt_BR |
DeCS | Mycobacterium fortuitum | pt_BR |
DeCS | Fagócitos | pt_BR |
DeCS | Organismos Livres de Patógenos Específicos | pt_BR |
DeCS | Camundongos | pt_BR |