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https://www.arca.fiocruz.br/handle/icict/32360
DEVELOPMENT OF AN URBAN MOLECULAR XENOMONITORING SYSTEM FOR LYMPHATIC FILARIASIS IN THE RECIFE METROPOLITAN REGION, BRAZIL
Aedes/growth & development
Aedes/parasitology
Aged
Animals
Antigens, Protozoan/blood
Brazil/epidemiology
Cross-Over Studies
Culex/growth & development
Disease Transmission, Infectious
Elephantiasis, Filarial/epidemiology
Culex/parasitology
Entomology/methods
Epidemiological Monitoring
Female
Humans
Immunoassay
Male
Middle Aged
Polymerase Chain Reaction
Prevalence
Urban Population
Wuchereria bancrofti/genetics
Aedes / crescimento e desenvolvimento
Aedes / parasitologia
Envelhecido
Idoso, 80 anos ou mais
Animais
Antígenos, Protozoários / sangue
Brasil / epidemiologia
Estudos Cross-Over
Culex / crescimento e desenvolvimento
Culex / parasitologia
Transmissão de Doenças Infecciosas
Filariose Linfática / epidemiologia
Entomologia / métodos
Monitorização Epidemiológica
Fêmea
Humanos
Imunoensaio
Masculino
Meia idade
Reação em Cadeia da Polimerase
Prevalência
População urbana
Wuchereria bancrofti / genética
Wuchereria bancrofti / isolamento e purificação
Author
Ramesh, Anita
Cameron, Mary
Spence, Kirstin
Hoek Spaans, Remy
Melo-Santos, Maria A. V.
Paiva, Marcelo H. S.
Guedes, Duschinka R. D.
Barbosa, Rosangela M. R.
Oliveira, Claudia M. F.
Sá, André
Jeffries, Claire L.
Castanha, Priscila M. S.
Oliveira, Paula A. S.
Walker, Thomas
Alexander, Neal
Braga, Cynthia
Cameron, Mary
Spence, Kirstin
Hoek Spaans, Remy
Melo-Santos, Maria A. V.
Paiva, Marcelo H. S.
Guedes, Duschinka R. D.
Barbosa, Rosangela M. R.
Oliveira, Claudia M. F.
Sá, André
Jeffries, Claire L.
Castanha, Priscila M. S.
Oliveira, Paula A. S.
Walker, Thomas
Alexander, Neal
Braga, Cynthia
Affilliation
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil / London School of Hygiene & Tropical Medicine (LSHTM). Department of Infectious Disease Epidemiology. London, United Kingdom.
LSHTM. Department of Disease Control. London, United Kingdom.
LSHTM. Department of Disease Control. London, United Kingdom.
LSHTM. Department of Disease Control. London, United Kingdom.
LSHTM. Department of Disease Control. London, United Kingdom.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Centro de Geoprocessamento e Estatística. Recife, PE, Brasil.
LSHTM. Department of Disease Control. London, United Kingdom.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil / Universidade de Pernambuco. Faculdade de Ciências Médicas. Instituto Biológico de Medicina. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil.
LSHTM. Department of Disease Control. London, United Kingdom.
London School of Hygiene & Tropical Medicine (LSHTM). Department of Infectious Disease Epidemiology. London, United Kingdom.
LSHTM. Department of Disease Control. London, United Kingdom.
LSHTM. Department of Disease Control. London, United Kingdom.
LSHTM. Department of Disease Control. London, United Kingdom.
LSHTM. Department of Disease Control. London, United Kingdom.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Centro de Geoprocessamento e Estatística. Recife, PE, Brasil.
LSHTM. Department of Disease Control. London, United Kingdom.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil / Universidade de Pernambuco. Faculdade de Ciências Médicas. Instituto Biológico de Medicina. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil.
LSHTM. Department of Disease Control. London, United Kingdom.
London School of Hygiene & Tropical Medicine (LSHTM). Department of Infectious Disease Epidemiology. London, United Kingdom.
Abstract
Introduction: Molecular xenomonitoring (MX)—pathogen detection in the mosquito rather than human— is a promising tool for lymphatic filariasis (LF) surveillance. In the Recife Metropolitan Region (RMR), the last LF focus in Brazil, Culex quinquefasciatus mosquitoes have been implicated in transmitting Wuchereria bancrofti parasites. This paper presents findings on
the ideal mosquito collection method, mosquito dispersion, W. bancrofti infection in mosquitoes and W. bancrofti antigen in humans to aid MX development. Methods: Experiments occurred within two densely populated urban areas of Olinda, RMR, in July and August 2015. U.S. Centers for Disease Control and Prevention (CDC) light traps were compared to battery-powered aspirators as collection methods, and mosquito dispersion
was measured by mosquito mark release recapture (MMRR). Female Cx. quinquefasciatus were tested by PCR for W. bancrofti infection, and study area residents were screened by rapid tests for W. bancrofti antigen. Results: Aspirators caught 2.6 times more total Cx. quinquefasciatus, including 38 times more bloodfed and 5 times more gravid stages, than CDC light traps. They also collected 123 times more Aedes aegypti. Of the 9,644 marked mosquitoes released, only ten (0.01%) were recaptured, nine of which were < 50m (34.8m median, 85.4m maximum) from the release point. Of 9,169 unmarked mosquitoes captured in the MMR, 38.3% were unfed, 48.8% blood-fed, 5.5% semi-gravid, and 7.3% gravid. PCR on 182 pools (1,556 mosquitoes) found no evidence of W. bancrofti infection in Cx. quinquefasciatus. Rapid tests on 110 of 111 eligible residents were all negative for W. bancrofti antigen. Conclusions: Aspirators were more effective than CDC light traps at capturing Ae. aegypti and all but
unfed stages of Cx. quinquefasciatus. Female Cx. quinquefasciatus traveled short (< 86m) distances in this urban area. Lack of evidence for W. bancrofti infection in mosquitoes and antigen in humans in these fine-scale studies does not indicate that LF transmission has ceased in the RMR. A MX surveillance system should consider vector-specific collection methods, mosquito dispersion, and spatial scale but also local context, environmental factors such as sanitation, and host factors such as infection prevalence and treatment history.
Keywords
AdultAedes/growth & development
Aedes/parasitology
Aged
Animals
Antigens, Protozoan/blood
Brazil/epidemiology
Cross-Over Studies
Culex/growth & development
Disease Transmission, Infectious
Elephantiasis, Filarial/epidemiology
Culex/parasitology
Entomology/methods
Epidemiological Monitoring
Female
Humans
Immunoassay
Male
Middle Aged
Polymerase Chain Reaction
Prevalence
Urban Population
Wuchereria bancrofti/genetics
DeCS
AdultoAedes / crescimento e desenvolvimento
Aedes / parasitologia
Envelhecido
Idoso, 80 anos ou mais
Animais
Antígenos, Protozoários / sangue
Brasil / epidemiologia
Estudos Cross-Over
Culex / crescimento e desenvolvimento
Culex / parasitologia
Transmissão de Doenças Infecciosas
Filariose Linfática / epidemiologia
Entomologia / métodos
Monitorização Epidemiológica
Fêmea
Humanos
Imunoensaio
Masculino
Meia idade
Reação em Cadeia da Polimerase
Prevalência
População urbana
Wuchereria bancrofti / genética
Wuchereria bancrofti / isolamento e purificação
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