Author | Corrêa, José R. | |
Author | Atella, Georgia C. | |
Author | Batista, Marcelo M. | |
Author | Soares, Maurilio J. | |
Access date | 2019-04-04T12:53:25Z | |
Available date | 2019-04-04T12:53:25Z | |
Document date | 2008 | |
Citation | CORRÊA, José R. et al. Transferrin uptake in Trypanosoma cruzi is impaired by interference on cytostome-associated cytoskeleton elements and stability of membrane cholesterol, but not by obstruction of clathrin-dependent endocytosis. Experimental Parasitology, v. 119, p. 58-66, Dec. 2008. | pt_BR |
ISSN | 0014-4894 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/32358 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Microscopia eletrônica de transmissão | pt_BR |
Subject in Portuguese | Endocitose | pt_BR |
Subject in Portuguese | Transferrina | pt_BR |
Subject in Portuguese | Citoesqueleto | pt_BR |
Subject in Portuguese | Citostome | pt_BR |
Subject in Portuguese | Colesterol da membrana | pt_BR |
Title | Transferrin uptake in Trypanosoma cruzi is impaired by interference on cytostome-associated cytoskeleton elements and stability of membrane cholesterol, but not by obstruction of clathrin-dependent endocytosis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.exppara.2007.12.010 | |
Abstract | Transferrin uptake by Trypanosoma cruzi epimastigotes occurs mainly through the cytostome/cytopharynx. Here, we present evidences for the association of sterol-rich membrane domains with the transferrin endocytic site. Assays using pharmacological treatments to disrupt clathrin-coated pits and hinder caveolae formation showed no association between transferrin uptake and clathrin-dependent endocytosis, but indicated that cholesterol stability in membrane domains is essential for the endocytosis of transferrin. Furthermore, it was observed a connection between the integrity of cytoskeleton elements at the cytopharynx and the function of the cytostome. Our data show that T. cruzi epimastigotes depend on a specialized pathway for transferrin uptake, which is cholesterol-dependent, clathrin-independent, and closely associated with the structural stability of the cytostome/cytopharynx cytoskeleton. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Parana. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Transmission electron microscopy | pt_BR |
Subject | Transferrin | pt_BR |
Subject | Endocytosis | pt_BR |
Subject | Cytoskeleton | pt_BR |
Subject | Membrane cholesterol | pt_BR |
Subject | Clathrin | pt_BR |
e-ISSN | 1090-2449 | |
Embargo date | 2022-01-01 | |