Author | Kasprowicz, Victoria | |
Author | Ward, Scott M. | |
Author | Turner, Alison | |
Author | Grammatikos, Alexandros | |
Author | Nolan, Brian E. | |
Author | Lewis-Ximenez, Lia | |
Author | Sharp, Charles | |
Author | Woodruff, Jenny | |
Author | Fleming, Vicki M. | |
Author | Sims, Stuart | |
Author | Walker, Bruce D. | |
Author | Sewell, Andrew K. | |
Author | Lauer, Georg M. | |
Author | Klenerman, Paul | |
Access date | 2019-02-26T14:51:06Z | |
Available date | 2019-02-26T14:51:06Z | |
Document date | 2008 | |
Citation | KASPROWICZ, Victoria; et al. Defining the directionality and quality of influenza virus–specific CD8+ T cell cross-reactivity in individuals infected with hepatitis C virus. Journal of Clinical Investigation, v.118, n.3, p.1143-1153, Mar. 2008. | pt_BR |
ISSN | 0021-9738 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31880 | |
Language | eng | pt_BR |
Publisher | American Society for Clinical Investigation | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Vírus Influenza | pt_BR |
Subject in Portuguese | CD+8 | pt_BR |
Subject in Portuguese | Células T | pt_BR |
Subject in Portuguese | Indivíduos infectados | pt_BR |
Subject in Portuguese | Vírus da hepatite C | pt_BR |
Title | Defining the directionality and quality of influenza virus-specific CD8+ T cell cross-reactivity in individuals infected with hepatitis C virus | pt_BR |
Type | Article | pt_BR |
DOI | 10.1172/JCI33082 | |
Abstract | Cross-reactivity of murine and recently human CD8(+) T cells between different viral peptides, i.e., heterologous immunity, has been well characterized. However, the directionality and quality of these cross-reactions is critical in determining their biological importance. Herein we analyzed the response of human CD8(+) T cells that recognize both a hepatitis C virus peptide (HCV-NS3) and a peptide derived from the influenza neuraminidase protein (Flu-NA). To detect the cross-reactive CD8(+) T cells, we used peptide-MHC class I complexes (pMHCs) containing a new mutant form of MHC class I able to bind CD8 more strongly than normal MHC class I complexes. T cell responses against HCV-NS3 and Flu-NA peptide were undetectable in normal donors. In contrast, some responses against the Flu-NA peptide were identified in HCV(+) donors who showed strong HCV-NS3-specific reactivity. The Flu-NA peptide was a weak agonist for CD8(+) T cells in HCV(+) individuals on the basis of novel pMHCs and functional assays. These data support the idea of cross-reactivity between the 2 peptides, but indicate that reactivity toward the Flu-NA peptide is highly CD8-dependent and occurs predominantly after priming during HCV infection. Our findings indicate the utility of the novel pMHCs in dissecting cross-reactivity and suggest that cross-reactivity between HCV and influenza is relatively weak. Further studies are needed to relate affinity and functionality of cross-reactive T cells. | pt_BR |
Affilliation | Partners AIDS Research Center and Infectious Disease Division. Massachusetts General Hospital and Harvard Medical School. Charlestown, Massachusetts, USA. | pt_BR |
Affilliation | Peter Medawar Building for Pathogen Research. Nuffield Department of Clinical Medicine. University of Oxford. Oxford, United Kingdom. | pt_BR |
Affilliation | Peter Medawar Building for Pathogen Research. Nuffield Department of Clinical Medicine. University of Oxford. Oxford, United Kingdom. | pt_BR |
Affilliation | Peter Medawar Building for Pathogen Research. Nuffield Department of Clinical Medicine. University of Oxford. Oxford, United Kingdom. | pt_BR |
Affilliation | Partners AIDS Research Center and Infectious Disease Division. Massachusetts General Hospital and Harvard Medical School. Charlestown, Massachusetts, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Peter Medawar Building for Pathogen Research. Nuffield Department of Clinical Medicine. University of Oxford. Oxford, United Kingdom. | pt_BR |
Affilliation | Peter Medawar Building for Pathogen Research. Nuffield Department of Clinical Medicine. University of Oxford. Oxford, United Kingdom. | pt_BR |
Affilliation | Peter Medawar Building for Pathogen Research. Nuffield Department of Clinical Medicine. University of Oxford. Oxford, United Kingdom. | pt_BR |
Affilliation | Peter Medawar Building for Pathogen Research. Nuffield Department of Clinical Medicine. University of Oxford. Oxford, United Kingdom. | pt_BR |
Affilliation | Partners AIDS Research Center and Infectious Disease Division. Massachusetts General Hospital and Harvard Medical School. Charlestown, Massachusetts, USA. | pt_BR |
Affilliation | University of Cardiff. Department of Medical Biochemistry and Immunology. Cardiff, United Kingdom. | pt_BR |
Affilliation | Partners AIDS Research Center and Infectious Disease Division. Massachusetts General Hospital and Harvard Medical School. Charlestown, Massachusetts, USA. | pt_BR |
Affilliation | Peter Medawar Building for Pathogen Research. Nuffield Department of Clinical Medicine. University of Oxford. Oxford, United Kingdom. | pt_BR |
Subject | Influenza virus | pt_BR |
Subject | CD+8 specific | pt_BR |
Subject | T cells | pt_BR |
Subject | Individuals infected | pt_BR |
Subject | Hepatitis C virus | pt_BR |
e-ISSN | 1558-8238 | |