Author | Robert, Anny Waloski | |
Author | Angulski, Addeli Bez Batti | |
Author | Spangenberg, Lucia | |
Author | Shigunov, Patrícia | |
Author | Pereira, Isabela Tiemy | |
Author | Bettes, Paulo Sergio Loiacono | |
Author | Naya, Hugo | |
Author | Dominguez, Alejandro Correa | |
Author | Dallagiovanna, Bruno | |
Author | Stimamiglio, Marco Augusto | |
Access date | 2019-02-20T19:47:52Z | |
Available date | 2019-02-20T19:47:52Z | |
Document date | 2018 | |
Citation | ROBERT, Anny Waloski et al. Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis. Scientific Reports, v. 8, n. 1, p. 1-11, 2018. | pt_BR |
ISSN | 2045-2322 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31797 | |
Language | por | pt_BR |
Publisher | Nature Research | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Células-tronco mesenquimais | pt_BR |
Title | Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/s41598-018-22991-6 | |
Abstract | Mesenchymal stem cells (MSCs) have been widely studied with regard to their potential use in cell therapy protocols and regenerative medicine. However, a better comprehension about the factors and molecular mechanisms driving cell differentiation is now mandatory to improve our chance to manipulate MSC behavior and to benefit future applications. In this work, we aimed to study gene regulatory networks at an early step of osteogenic differentiation. Therefore, we analyzed both the total mRNA and the mRNA fraction associated with polysomes on human adipose tissue-derived stem cells (hASCs) at 24 h of osteogenesis induction. The RNA-seq results evidenced that hASC fate is not compromised with osteogenesis at this time and that 21 days of continuous cell culture stimuli are necessary for full osteogenic differentiation of hASCs. Furthermore, early stages of osteogenesis induction involved gene regulation that was linked to the management of cell behavior in culture, such as the control of cell adhesion and proliferation. In conclusion, although discrete initial gene regulation related to osteogenesis occur, the first 24 h of induction is not sufficient to trigger and drive in vitro osteogenic differentiation of hASCs. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto Pasteur. Unidade de Bioinformática. Montevideo, Uruguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Cirurgia Plástica Dr. Paulo Bettes. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto Pasteur. Unidade de Bioinformática. Montevideo, Uruguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Subject | Mesenchymal Stromal Cells | pt_BR |
Subject | Mesenchymal stem cells | pt_BR |
Subject | Osteogenesis | pt_BR |
Subject | Gene Expression Regulation | pt_BR |
Subject in Spanish | Células del Estroma Mesenquimatoso | pt_BR |
Subject in Spanish | Osteogénesis | pt_BR |
Subject in Spanish | Regulación de la Expresión Génica | pt_BR |
DeCS | Células Mesenquimais Estromais | pt_BR |
DeCS | Osteogênese | pt_BR |
DeCS | Regulação da Expressão Gênica | pt_BR |