Author | Miranda, Luisa Helena Monteiro de | |
Author | Meli, Marina | |
Author | Silva, Fátima Conceição | |
Author | Novacco, Marilisa | |
Author | Menezes, Rodrigo Caldas | |
Author | Pereira, Sandro Antonio | |
Author | Sugiarto, Sarah | |
Author | Reis, Érica Guerino dos | |
Author | Gremião, Isabella Dib Ferreira | |
Author | Hofmann-Lehmann, Regina | |
Access date | 2019-02-15T16:14:14Z | |
Available date | 2019-02-15T16:14:14Z | |
Document date | 2018 | |
Citation | MIRANDA, Luisa Helena Monteiro de et al. Co-infection with feline retrovirus is related to changes in immunological parameters of cats with sporotrichosis. Plos One, v. 13, n. 11, p. 1-16, Nov. 2018. | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31683 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | |
Subject in Portuguese | Esporotricose | pt_BR |
Subject in Portuguese | Co-infecção | pt_BR |
Subject in Portuguese | Parâmetros imunológicos | pt_BR |
Subject in Portuguese | Gatos | pt_BR |
Subject in Portuguese | Retrovírus felino | pt_BR |
Title | Co-infection with feline retrovirus is related to changes in immunological parameters of cats with sporotrichosis | pt_BR |
Type | Article | |
DOI | 10.1371/journal.pone.0207644 | |
Abstract | Feline sporotrichosis due to Sporothrix brasiliensis is frequently severe and often correlated to zoonotic transmission. Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) cause immunodeficiency in cats; no association has been identified with critical cases of sporotrichosis. Moreover, the cytokine profile in Sporothrix-infected cats and a potential impact of retrovirus co-infections on their immunity is unknown. This study assessed immunological parameters in cats with sporotrichosis with and without FIV or FeLV co-infection. FeLV infection was detected by antigen ELISA and by provirus PCR. FIV infection was investigated through ELISA and Western blot. Cytokine transcription (IFN-γ, IL-4, IL-5, IL-6, IL-10, IL-12, TNF-α) was quantified using RT-qPCR and lymphocyte subpopulations (CD4, CD8, CD5 and CD21) were assessed by flow cytometry. Thirty cats with sporotrichosis were recruited to the study, including three FIV-positive and five FeLV-positive (progressive infection) cats. One cat with regressive FeLV infection was excluded from statistics. In comparison to retrovirus-negative cats, FIV-positive cats and FeLV-positive cats had higher IL-10 levels, FeLV-positive cats had lower IL-4 levels and FIV-positive cats had lower IL-12 levels and a lower CD4+/CD8+ ratio. Remarkably, all cats with poor general condition were FeLV (progressive infection) or FIV-positive, but the retrovirus status was not associated with the sporotrichosis treatment length or outcome. The immunological changes and the more severe clinical presentation observed in cats with retrovirus co-infections encourage future prospective studies that address the impact of these changes on prognostic determinants of feline sporotrichosis and the development of new therapy strategies that control disease spread. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil / University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland. | pt_BR |
Affilliation | University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Zurich. Vetsuisse Faculty. Clinical Laboratory and Center for Clinical Studies. Zurich, Switzerland. | pt_BR |
Subject | Co-infection | pt_BR |
Subject | Feline retrovirus | pt_BR |
Subject | Immunological parameters | pt_BR |
Subject | Sporotrichosis | pt_BR |
Subject | Cats | pt_BR |