Author | Menezes, Francisca Gleire R. | |
Author | Barbosa, Wescley E. | |
Author | Vasconcelos, Lorrana S. | |
Author | Rocha, Rafael S. | |
Author | Maggioni, Rodrigo | |
Author | Sousa, Oscarina V. | |
Author | Hofer, Ernesto | |
Author | Vieira, Regine H. S. F. | |
Access date | 2019-01-31T15:25:41Z | |
Available date | 2019-01-31T15:25:41Z | |
Document date | 2018 | |
Citation | MENEZES, Francisca Gleire R. et al. Genotypic assessment of a dichotomous key to identify Vibrio coralliilyticus, a coral pathogen. Dis. Aquat. DOrg., v.128, p.87-92, Mar. 2018. | pt_BR |
ISSN | 0177-5103 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31361 | |
Language | eng | pt_BR |
Publisher | Inter Research | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Vibrio coralliilyticus | pt_BR |
Subject in Portuguese | Genótipos | pt_BR |
Subject in Portuguese | Fenótipos | pt_BR |
Subject in Portuguese | Doença de corais | pt_BR |
Subject in Portuguese | Vibrioses | pt_BR |
Title | Genotypic assessment of a dichotomous key to identify Vibrio coralliilyticus, a coral pathogen | pt_BR |
Type | Article | pt_BR |
DOI | 10.3354/dao03209 | |
Abstract | Vibrio coralliilyticus is a known pathogen to corals and larvae of bivalves. Its identification
is made based on phenotypic and genotypic characters of isolated strains. To evaluate the
efficiency of the phenotypic identification, 21 strains identified as V. coralliilyticus using a widely
used dichotomous key were analyzed by qualitative PCR and sequencing of the 16S rDNA region.
The results obtained by the behavioral test, amino acids usage, allow us to distinguish 3 A/L/O
profiles: (1) A+/L−/O+; (2) A+/L+/O+; and (3) A−/L+/O+. In the genotypic tests, all strains tested
positive with primers specific for the Vibrio genus. However, when primers were used for species
identification, the results did not match those obtained with the dichotomous key chosen. The
phenotypic characteristics taken into account to set apart V. coralliilyticus and other species were
not proven to be efficient. More information about the morphological diversity of colonies and
enzymatic activities should be considered in the formulation of phenotypic keys for V. coralliilyticus
and related species. | pt_BR |
Affilliation | Universidade Federal do Ceará. Departamento de Engenharia de Pesca. Fortaleza, CE, Brasil. | pt_BR |
Affilliation | Universidade Federal do Ceará. Departamento de Engenharia de Pesca. Fortaleza, CE, Brasil. | pt_BR |
Affilliation | Universidade Federal do Ceará. Instituto de Ciências Marinhas. Fortaleza, CE, Brasil. | pt_BR |
Affilliation | Universidade Federal do Ceará. Departamento de Engenharia de Pesca. Fortaleza, CE, Brasil. | pt_BR |
Affilliation | Universidade Federal do Ceará. Instituto de Ciências Marinhas. Fortaleza, CE, Brasil. | pt_BR |
Affilliation | Universidade Federal do Ceará. Instituto de Ciências Marinhas. Fortaleza, CE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Ceará. Departamento de Engenharia de Pesca. Fortaleza, CE, Brasil / Universidade Federal do Ceará. Instituto de Ciências Marinhas. Fortaleza, CE, Brasil. | pt_BR |
Subject | Vibriosis | pt_BR |
Subject | Coral disease | pt_BR |
Subject | Phenotype | pt_BR |
Subject | Genotype | pt_BR |
Subject | Vibrio coralliilyticus | pt_BR |
e-ISSN | 1616-1580 | |
Embargo date | 2030-01-01 | |