Author | Vasconcelos, Tassia Cristina Bello de | |
Author | Doyen, Noelle | |
Author | Cavaillon, Jean-Marc | |
Author | Bruno, Sávio Freire | |
Author | Campos, Monique Paiva de | |
Author | Miranda, Luisa Helena Monteiro de | |
Author | Madeira, Maria de Fátima | |
Author | Belo, Vinícius Silva | |
Author | Figueiredo, Fabiano Borges | |
Access date | 2019-01-22T10:27:57Z | |
Available date | 2019-01-22T10:27:57Z | |
Document date | 2016 | |
Citation | VASCONCELOS, Tassia Cristina Bello de et al. Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features. Veterinary Parasitology, v. 227, p. 8-14, 2016. | pt_BR |
ISSN | 0304-4017 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31203 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | Cytokine and iNOS profiles in lymph nodes of dogs naturally infected with Leishmania infantum and their association with the parasitic DNA load and clinical and histopathological features | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.vetpar.2016.07.017 | |
Abstract | In South America, visceral leishmaniasis is a zoonotic disease with severe evolution characteristics in humans, and dogs are its main reservoir. In this context, this study aimed to evaluate the clinical status of dogs from a Brazilian endemic area naturally, at Barra Mansa municipality, infected with Leishmania infantum, in conjunction with their histopathological profile and, in order to determine possible markers of susceptibility or resistance to the disease, parasitic DNA load, cytokine and iNOS mRNA expression profiles were investigated in lymph nodes. High levels of IFN-ɣ and IL-6 mRNA were detected. Both IFN-ɣ and IL-6 mRNA were associated with disorganization of the corticomedullary region. IFN-ɣ and TNF-α mRNA were associated with the absence of follicular hyperplasia. The regulatory pathway was remarkable with IL-10 mRNA detection and its significant association with the severity of the disease. Plasmacytosis and sinus histiocytosis were associated with high loads of parasitic DNA, but there was no significant association between the parasite DNA load and animal clinical alterations. Since high parasitic loads were found in animals with or without symptoms, clinical examination cannot be considered as a criterion for disease susceptibility assessment. | pt_BR |
Affilliation | Universidade Federal Fluminense. Pós-graduação em Medicina Veterinária. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Institut Pasteur. Unit Cytokines and Inflammation. Department of Infection & Epidemiology. Paris, France. | pt_BR |
Affilliation | Institut Pasteur. Unit Cytokines and Inflammation. Department of Infection & Epidemiology. Paris, France. | pt_BR |
Affilliation | Universidade Federal Fluminense. Pós-graduação em Medicina Veterinária. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal de São João del-Rei. Campus Centro-Oeste “Dona Lindu”. Divinópolis, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Visceral leishmaniasis | pt_BR |
Subject | Immunopathology | pt_BR |
Subject | Canine infection | pt_BR |
e-ISSN | 1873-2550 | |