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https://www.arca.fiocruz.br/handle/icict/30638
HIGHLY EXPRESSED PLACENTAL MIRNAS CONTROL KEY BIOLOGICAL PROCESSES IN HUMAN CANCER CELL LINES
Author
Affilliation
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Barretos Cancer Hospital. Molecular Oncology Research Center. Barretos, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
Ribeirão Preto Medical School, University of São Paulo. Department of Gynecology and Obstetrics. Ribeirão Preto, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Center for Medical Genomics. Ribeirão , SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil.
Ribeirão Preto Medical School, University of São Paulo. Department of Gynecology and Obstetrics. Ribeirão Preto, SP, Brazil.
University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem Cell and Cell Therapy. Center for Cell-Based Therapy. Riberão Preto, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Center for Medical Genomics. Ribeirão , SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil.
Abstract
Despite being a healthy tissue, the constituent cells of the placenta, share
similar characteristics with tumor cells, such as increased cell growth, migration, and
invasion. However, while these processes are stochastic and uncontrolled in cancer
cells, in placenta they are precisely controlled. Since miRNAs have been reported to
regulate genes that control the molecular mechanisms necessary for the development
of both human placenta and cancer, we addressed for miRNAs highly expressed in the
placenta that could be involved in tumorigenesis. Here, we assessed the miRNA profile
in placenta samples using microarray analysis. The results showed that miR-451 and
miR-720, highly expressed placental miRNAs, presented very low or undetectable
expression in cancer cell lines compared to the normal placenta and healthy tissues.
Additionally, transfection of miR-451 or miR-720 mimics in choriocarcinoma cell line
(JEG3) and colorectal adenocarcinoma cell line (HT-29) resulted in impaired cell
proliferation, decreased cell migration and invasion and reduced ability of colony
formation. These findings provide evidence that placenta may work as an alternative
model to identify novel miRNAs involved in pathways controlling tumorigenesis.
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