Author | Mesquita, Emersom C. | |
Author | Hottz, Eugenio D. | |
Author | Amancio, Rodrigo T. | |
Author | Carneiro, Alan B. | |
Author | Palhinha, Lohanna | |
Author | Coelho, Lara E. | |
Author | Grinsztejn, Beatriz | |
Author | Zimmerman, Guy A. | |
Author | Rondina, Matthew T. | |
Author | Weyrich, Andrew S. | |
Author | Bozza, Patrícia T. | |
Author | Bozza, Fernando A. | |
Access date | 2018-12-03T15:25:04Z | |
Available date | 2018-12-03T15:25:04Z | |
Document date | 2018 | |
Citation | MESQUITA, Emersom C. et al. Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals. Scientific Reports, v. 8, n. 1, p. 1-10, 2018. | pt_BR |
ISSN | 2045-2322 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/30361 | |
Language | eng | pt_BR |
Publisher | Nature Research | pt_BR |
Rights | open access | pt_BR |
Title | Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/s41598-018-33403-0 | |
Abstract | Cardiovascular diseases and thrombotic events became major clinical problems in the combined antiretroviral therapy (cART) era. Although the precise mechanisms behind these clinical problems have not been fully elucidated, a persistent pro-inflammatory state plays a central role. As platelets play important roles on both, thrombus formation and inflammatory/immune response, we aimed at investigating platelet function in HIV-infected subjects virologically controlled through cART. We evaluate parameters of activation, mitochondrial function and activation of apoptosis pathways in platelets from 30 HIV-infected individuals under stable cART and 36 healthy volunteers. Despite viral control achieved through cART, HIV-infected individuals exhibited increased platelet activation as indicated by P-selectin expression and platelet spreading when adhered on fibrinogen-coated surfaces. Platelets from HIV-infected subjects also exhibited mitochondrial dysfunction and activation of apoptosis pathways. Finally, thrombin stimuli induced lower levels of P-selectin translocation and RANTES secretion, but not TXA2 synthesis, in platelets from HIV-infected individuals compared to control; and labeling of platelet alpha granules showed reduced granule content in platelets from HIV-infected individuals when compared to healthy subjects. In summary, platelets derived from HIV-infected individuals under stable cART exhibit a phenotype of increased activation, activation of the intrinsic pathway of apoptosis and undermined granule secretion in response to thrombin. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil./ Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | University of Utah. Molecular Medicine Program and Department of Internal Medicine. Salt Lake City, Utah, USA. | pt_BR |
Affilliation | University of Utah. Molecular Medicine Program and Department of Internal Medicine. Salt Lake City, Utah, USA. | pt_BR |
Affilliation | University of Utah. Molecular Medicine Program and Department of Internal Medicine. Salt Lake City, Utah, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil / Instituto D'OR de Pesquisa e Ensino. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | HIV | pt_BR |
Subject | Antiretroviral therapy | pt_BR |
Subject | Platelet | pt_BR |
Subject | cART | pt_BR |