Author | Rocha, Eliseu Soares de Oliveira | |
Author | Oliveira, Jaquelline Germano de | |
Author | Santos, João Rodrigues dos | |
Author | Rodrigues, Gisele Olinto Libânio | |
Author | Figueiredo, Leandra Barcelos | |
Author | Pessanha, José Eduardo Marques | |
Author | Proietti, Fernando Augusto | |
Author | Fonseca, Flávio Guimarães da | |
Author | Bonjardim, Claudio Antonio | |
Author | Ferreira, Paulo Cesar Peregrino | |
Author | Kroon, Erna Geessien | |
Access date | 2018-10-29T14:03:07Z | |
Available date | 2018-10-29T14:03:07Z | |
Document date | 2013 | |
Citation | ROCHA, Eliseu Soares de Oliveira et al. Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection. Journal of Virological Methods, v. 187, n. 1, p. 114-120, 2013 | pt_BR |
ISSN | 0166-0934 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/29776 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Dengue vírus | pt_BR |
Subject in Portuguese | sorologia | pt_BR |
Subject in Portuguese | diagnostico | pt_BR |
Subject in Portuguese | ELISA | pt_BR |
Title | Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jviromet.2012.09.012 | |
Abstract | Dengue virus (DENV) is the most prevalent arbovirus in the world, found mainly in tropical regions. As clinical manifestations present frequently as nonspecific febrile illness, laboratory diagnosis is essential to confirm DENV infections and for epidemiological studies. Recombinant envelope (E) antigens of four serotypes of DENV were used to develop an immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA). To evaluate the IgG-ELISA, a panel of serum samples that had been tested previously by a plaque reduction neutralization test (PRNT) was investigated for the presence of anti-E antibodies against the four DENV serotypes. IgG-ELISA was found to have a sensitivity (91%) and specificity (98%) at a receiver-operating characteristic (ROC) optimized cutoff and demonstrated high performance as well as good indexes. A concordance of 97% was achieved between both assays, and only 21/704 (3%) samples were not concordant. The results of the present study demonstrate a moderate correlation between neutralizing antibody titers and IgG-ELISA values. These findings indicate that the recombinant protein-based IgG-ELISA is a suitable method for routine serodiagnosis, monitoring and seroepidemiological studies of DENV infections. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Prefeitura Municipal de Belo Horizonte. Secretaria Municipal de Saúde. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Preventiva e Social. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Virologia Comparada. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Vírus. Belo Horizonte, MG, Brazil | pt_BR |
Subject | Dengue vírus | pt_BR |
Subject | Serology | pt_BR |
Subject | Diagnosis | pt_BR |
Subject | ELISA | pt_BR |
Embargo date | 2020-01-01 | |