Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/29353
CHARACTERIZING SUBPOPULATIONS OF NEOPLASTIC CELLS IN SEROUS EFFUSIONS. THE ROLE OF IMMUNOCYTOCHEMISTRY
Affilliation
Federal University of Bahia. Department of Gynecology, Obstetrics and Human Reproduction. Salvador, BA, Brazil
Federal University of Bahia. Department of Anatomic Pathology and Immunology. Salvador, BA, Brazil
Universidade Estadual Paulista. Department of Pathology. São Paulo, SP, Brazil
Federal University of Bahia. Department of Anatomic Pathology and Immunology. Salvador, BA, Brazil
Universidade Estadual Paulista. Department of Pathology. São Paulo, SP, Brazil
Abstract
To analyze the role of immunochemistry in serous effusions. STUDY DESIGN: We analyzed cell blocks of 18 pleural
and 18 peritoneal effusions
diagnosed as malignant (18),
benign (14) and suspicious
(4). They were immunostained
by the avidin-biotin
complex method with a panel
of four monoclonal antibodies—
CEA, Ber-EP4, LeuM1
(CD15) and p53—and, for
lectins (Ulex europaeus)
UEA-l, ConA and ConBr.
RESULTS: Seventeen of the
18 cases of adenocarcinoma were positive for CEA
(95%), 12 (66.6%) for Ber-EP4, 11 (61%) for CD15 and
11 (61%) for p53. Twelve of the 18 (66.6%) were positive
for UEA-1, CEA, Ber-EP4 and CD15. UEA-1 did not
react with mesothelial cells. p53 Gave a positive reaction
in only one case, reactive mesothelial cells. ConA and
ConBr reacted indiscriminately with benign and malignant
cells; thus, it was not useful in distinguishing between
these cells.
CONCLUSION: In this context no antibody used alone
is reliable for corroborating a diagnosis, but the selective
use of a small panel of three markers (CEA, Ber-EP4 and
LeuM1) can be very useful in solving diagnostic difficulties
in the cytodiagnosis of
serous effusions.
Share