Author | Cruz, Wagner Baetas da | |
Author | Alves, Lucinéia | |
Author | Guimarães, Erick V. | |
Author | Santos-Silva, Alessandra | |
Author | Pessolani, Maria Cristina V. | |
Author | Barbosa, Helene S. | |
Author | Corte-Real, Suzana | |
Author | Cavalcante, Leny A. | |
Access date | 2018-09-20T15:41:05Z | |
Available date | 2018-09-20T15:41:05Z | |
Document date | 2009 | |
Citation | CRUZ, Wagner Baetas da; et al. Efficient uptake of mannosylated proteins by a human Schwann cell line. Histology and Histopathology, v.24, p.1029-1034, 2009. | pt_BR |
ISSN | 0213-3911 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28943 | |
Language | eng | pt_BR |
Publisher | Universidad de Murcia | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Receptores de reconhecimento de padrões | pt_BR |
Subject in Portuguese | Receptor de manose | pt_BR |
Subject in Portuguese | Compartimentos endocíticos | pt_BR |
Title | Efficient uptake of mannosylated proteins by a human Schwann cell line | pt_BR |
Type | Article | pt_BR |
Abstract | Complex carbohydrate structures are essential molecules of infectious microbes and host cells, and are involved in cell signaling associated with inflammatory and immune responses. The uptake of mannose-tailed glycans is usually carried out by macrophages, dendritic cells (DCs), and other professional phagocytes to trigger MHC class I- and MHC class II-restricted antigen presentation, and to promote T cell effector responses. Since Schwann cells (SCs) have been proposed as immunocompetent cells, we investigated whether a human cell line (ST88-14 cells) could bind mannosylated ligands in a specific manner. The saturation of uptake of mannosylated molecules by ST88-14 cells and the internalization and distribution pathway of these ligands were tested by cytometry and confocal plus electron microscopy, respectively. This uptake showed a dose-dependent increase, the saturation point being reached at high concentrations of mannosyl residues/240 mM mannose. Merging of man/BSA-FITC and S100 labeling showed their partial, but, significant colocalization. Ultrastructural analysis of ST88-14 cells after incubation with HRP-colloidal gold, without or with subsequent chasing at 37C, showed an initial location on the cell surface and temperature- and time-dependent internalization of the probe. Our findings suggest an efficient mannosylated ligand uptake system through putative lectin(s) that may be operational in inflammatory and immune responses. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia do Desenvolvimento. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia do Desenvolvimento. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia do Desenvolvimento. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Pattern recognition receptors | pt_BR |
Subject | Mannose receptor | pt_BR |
Subject | Endocytic compartments | pt_BR |
e-ISSN | 10.14670/HH-24.1029 | |